| Bcl-2反义寡核苷酸对肾癌细胞株的生长抑制作用 |
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| 引用本文： | 高江平,朱捷,杨素霞,陈萍,殷常康,洪宝发,李炎唐.Bcl-2反义寡核苷酸对肾癌细胞株的生长抑制作用[J].军医进修学院学报,2004,25(1):63-65. |
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| 作者姓名： | 高江平 朱捷 杨素霞 陈萍 殷常康 洪宝发 李炎唐 |
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| 作者单位： | 1. 解放军总医院,泌尿外科,北京,100853
2. 临沂市铁路医院,泌尿外科,山东,276000 |
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| 摘 要： | 目的：研究bcl-2反义寡核苷酸对肾细胞癌细胞的生长抑制作用及剂量一效应关系。方法：设计、合成与已知人类基因无同源性的bcl-2反义寡核苷酸(AS1翻译起始端，AS2编码区)，以阳离子脂质体Lipofectin转染不同的肾癌细胞株，MTS比色实验测定细胞活率。结果：单纯Lipofectin、反义寡核苷酸及正义寡核苷酸处理，对肾癌细胞ACHN生长无抑制作用，而转染入细胞内的反义寡核苷酸可抑制多种肾癌细胞的生长(ACHN、RCW和OSRC-2)，且抑制作用与剂量呈正相关。结论：bcl-2反义寡核苷酸可剂量依赖性抑制多种肾癌细胞的生长。
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| 关 键 词： | Bcl-2 反义寡核苷酸 肾癌细胞株 生长抑制作用 肿瘤 血清 |
| 文章编号： | 1005-1139(2004)01-0063-03 |
| 修稿时间： | 2003年6月18日 |
Effect of bcl-2 antisense oligodeoxynucleotides on human renal cell carcinoma cells growth |
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| GAO Jiang-ping,ZHU Jie,YANG Su-xia,CHEN Ping,SHI Li-xin,HONG Bao-fa,LI Yen-tang.Effect of bcl-2 antisense oligodeoxynucleotides on human renal cell carcinoma cells growth[J].Academic Journal of Pla Postgraduate Medical School,2004,25(1):63-65. |
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| Authors: | GAO Jiang-ping ZHU Jie YANG Su-xia CHEN Ping SHI Li-xin HONG Bao-fa LI Yen-tang |
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| Abstract: | Objective: To evaluate the effect and dose-response relationship of antisense oligodeoxynucleotides(ODNs) on inhibition of renal cell carcinoma growth in vitro. Methods: Design and synthesize antisense targeting various regions of the bcl-2 mRNA without the homology of the human genes. ODNs were delivered to human renal cell carcinoma cells in the form of complexes with liposome. The cytotoxicity of ODNs in RCC cells was determined by measurement of cell viability by use colorimetric assay.Results: Treatment with transfected antisense bcl-2 ODNs inhibited the growth of all tested RCC cells, but Liposome or ODNs alone. Inhibition by antisense bcl-2 ODNs of ACHN cells was dose dependent. Conclusions: Transfected ODNs effectively reduced the viability of many kinds of RCC cell in dose-dependent manner. |
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| Keywords: | renal cell carcinoma bcl-2 antisense oligonucleotides |
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