免疫球蛋白kappa轻链基因在鼻咽癌中表达的初步研究

目的：为了探讨Ｉｇｋ是否参与了鼻咽癌的癌变过程及可能机制，对Ｉｇｋ在鼻咽癌中的表达进行了研究，并初步探讨Ｉｇｋ与ＥＢ病毒潜伏膜蛋白基因ＬＭＰ－１在鼻咽癌中的相关性。方法：分别运用原位杂交技术和免疫组化技术检测鼻咽癌活检组织中Ｉｇｋ的ＲＮＡ和蛋白质水平的表达。Ｗｅｓｔｅｒｎ印迹分析两株鼻咽癌细胞系（ＣＮＥ１和ＣＮＥ＿ＣＭＰ）ｋａｐｐａ蛋白的表达及强度差异。结果：原位杂交技术显示１００％（４２例／４２

EXPRESSION OF IMMUNOGLUBULIN KAPPA LIGHT CHAIN IN NASOPHARYNGEAL CARCINOMA

Objective:To present evidence that kappa light chain gene expresses in both cell lines and biopsies of NPC and to determine whether kappa gene is related with Epstein Barr virus latent membrane protein 1 (LMP 1) in nasopharyngeal carcinogenesis.Methods:Kappa light chain expressed in NPC was detected by in situ hybridization, immunochemistry and Western blot. Results: All the samples,including 10 cases of well differentiated and 32 cases of poorly differentiated nasopharyngeal carcinoma,expressed kappa RNA and all the cases (7 cases) showed weak signals in the immunochemical test with anti free kappa light chain antibody.In addition, NPC cell line which was transfected with Epstein Bar virus latent membrane protein (designated CNe LMP cell line) seemed to express higher level of kappa free light chain than that without LMP transfection (designated CNE1). Conclusion:Immunoglobulin kappa gene may be involved in carcinogenesis of nasopharyngeal carcinoma through cooperation with EBV LMP.Therefore,to some extent,our studies have provided another possible molecular mechanism for nasopharyngeal carcinogenesis.