Composition of TCR-CD3 complex in human intestinal intraepithelial lymphocytes: lack of Fc{varepsilon}Rl{gamma} chain期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Composition of TCR-CD3 complex in human intestinal intraepithelial lymphocytes: lack of Fc{varepsilon}Rl{gamma} chain

Authors:	Balk, Steven P. Polischuk, John E. Probert, Christopher Stevens, Christopher Ebert, Ellen She, Jian Terhorst, Cox Blumberg, Richard S.

Affiliation:	Hematology/Oncology Division, Beth Israel Hospital, Harvard Medical School Boston, MA 02215, USA ¹ Gastroenterology Division, Brigham and Women's Hospital, Harvard Medical School Boston, MA 02115, USA ² Gastroenterology Division, Beth Israel Hospital, Harvard Medical School Boston, MA 02215, USA ³ Gastroenterology Division, Robert Wood Johnson Medical School New Brunswick, NJ 08903, USA ⁴ Division of Immunology, Beth Israel Hospital and Harvard Medical School Boston, MA 02215, USA

Abstract:	Human intestinal intraepithelial lymphocytes (DEL) are a uniquepopulation of predominantly CD8 ${alpha}$ ß⁺ TCR ${alpha}$ ß⁺lymphocytes and, to a lesser extent, TCR ${gamma}$ ${delta}$ ⁺ lymphocytes that proliferatepoorly to anti-CD3 mitogenic signals but display significantcytolytic activity. Studies in mouse model systems have shownthat the ${gamma}$ chain of the high-CD3 affinity receptor for IgE (Fc ${varepsilon}$ Rl ${gamma}$ )may substitute for the ${zeta}$ chain in the TCR-CD3 complex of iIEL.This has suggested that the functional properties of these cellsmay be associated with an altered composition of the TCR-CD3complex. We therefore analyzed the TCR-CD3 complex of normalhuman iIEL. One-and two-dimensional non-reducing/reducing SDS-PAGEanalysis of CD3 ${gamma}$ , CD3 ${delta}$ , CD3 ${varepsilon}$ , ${zeta}$ and Fc ${varepsilon}$ Rr ${gamma}$ chain immunopreclpitatesof cell surface radiolabeled proteins with subunit-specificantibodies revealed a TCR-CD3 complex without associated Fc ${varepsilon}$ Rr ${gamma}$ chains. Thus, normal human NEL contain a TCR-CD3 complex thatconsists predominantly of ${zeta}$ , homodimers in association with theß ${alpha}$ TCR and CD3 ${gamma}$ , ${delta}$ and ${varepsilon}$ , similar to the majority of peripherallymphocytes. This indicates that the distinct properties ofhuman DEL are not associated with substitutions of the Fc ${varepsilon}$ Rl ${gamma}$ chain in the TCR-CD3 complex.

Keywords:	epithelium FcR proliferation
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