西安地区高ALT值献血者HEV亚临床感染情况调查

目的探讨西安地区无偿献血者中戊型肝炎病毒的亚临床感染情况及其与ALT水平的相关性。方法收集西安地区2009年1月～2010年12月ALT水平升高的1874名无偿献血者血样，根据ALT检测结果分组后进行抗-HEV IgM和IgG、HEV RNA检测，比较各项指标间的相关性，并对HEV RNA阳性标本进行测序和基因分型。结果1874名无偿献血者中有7例抗-HEV IgM阳性，占0．37％；21例抗-HEV IgG阳性，占1．12％；8例HEV RNA阳性，占0．43％。其中7例抗-HEV IgM阳性者其RNA均为阳性。抗-HEV IgG的阳性率随年龄升高而增加。扩增8例HEV RNA阳性血样的保守序列ORF2分析发现5例基因型为Ⅰ型，另外3例基因型为Ⅳ型。结论西安地区戊型肝炎病毒的亚临床感染率为0．37％，HEV流行株主要为Ⅰ型和Ⅳ型病毒株。

Study on Hepatitis E Virus Subclinical Infection in High Alanine Aminotransferase Donors in Xi'an area

Objective To study the subclinical infection of hepatitis E virus of blood donors in Xi＇an area, and its correlation with ALT levels. Methods To collect 1 874 blood donors samples with high ALT levels in Xi＇an area from January 2009 to December 2010 and to test ALT. They were grouped according to the level of ALT. And they were detected on anti-HEV IgM and IgG, HEV RNA,then the relationships were analyzed among different indicators. The age of the donors, sequence and genotype were analyzed on the positive samples with HEV RNA. Results Among 1 874 blood donors, 7 were positive with HEV-IgM, accounting for 0. 37% ,all of the seven were positive with HEV RNA. 21 were positive with HEV-IgG, accounting for 1.12%. Anti-HEV IgG positive rate increased with the age of the dolors. 8 were positive with HEV RNA, accounting for 0. 43%. HEV RNA ORF2 was amplified in 8 samples, 5 belonged to genotype Ⅰ and the other 3 belonged to genotype Ⅳ. Conclusion The rate of subclinical infection of hepatitis E virus in Xitan was 0.37% ,the genotypes of hepatitis E belonged to genotype 1 and 4.