Interleukin 2 induces appearance of LGL/Leu11+/K562 lytic cells in Leu11- low density peripheral blood mononuclear cell population |
| |
| Authors: | A Lange H D Flad M Ernst A Jacak B Jazwiec W Scholz A J Ulmer |
| |
| Affiliation: | 1. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland;2. Research Institute Borstel, Borstel, F.R.G.;1. College of Marine Science and Technology, China University of Geosciences, Wuhan 430074, China;2. Hubei Key Laboratory of Marine Geological Resources, China University of Geosciences, Wuhan 430074, China;3. Engineering Research Centre of Rock-Soil Drilling & Excavation and Protection, Ministry of Education, Faculty of Engineering, Wuhan 430074, China;4. Geotechnical Research Centre, Department of Civil and Environmental Engineering, The University of Western Ontario, London, ON N6A 5B9, Canada;1. School of Mechanical Engineering, Chonnam National University, Gwangju 61186, Republic of Korea;2. Advanced Medical Device Research Center for Cardiovascular Disease, Chonnam National University, Gwangju 61186, Republic of Korea;3. Center for Next-Generation Sensor Research and development, Chonnam National University, Gwangju 61186, Republic of Korea |
| |
| Abstract: | Low density Percoll fraction cells cultured with interleukin 2 (IL-2) showed a higher proportion of large granular lymphocytes (LGL) and higher K562 cytolytic activity, as compared to a culture lacking IL-2. Furthermore, in a negatively selected Leu11- population, derived from low density cells, cultured for 7 days in medium supplemented with lymphocyte (L) or recombinant (R) IL-2, there appeared LGL and Leu11+ cells. Moreover, some level of K562 lytic activity and higher proportion of DR+ and Tac+ cells was found as compared to lacking IL-2 culture. Cytofluorograph analysis of cells labelled with propidium iodide revealed that a proportion of the low density Leu11- starting cell population entered the growth cycle while cultured with IL-2. In addition was found that Leu11+ cells evolve during culture with IL-2 into population lacking in part this phenotype marker. The present work shows that precursors of K562 cytolytic cells lacking Leu11 antigen reside in low density cell fraction, and that they may differentiate in LGL/Leu11+ cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
