| PCR-RFLP分析人结直肠癌DCC基因杂合性缺失 |
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| 引用本文： | 徐文怀,高随宽,万远廉,刘占兵,李晓武,王东民. PCR-RFLP分析人结直肠癌DCC基因杂合性缺失[J]. 北京大学学报(医学版), 1997, 0(1) |
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| 作者姓名： | 徐文怀 高随宽 万远廉 刘占兵 李晓武 王东民 |
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| 作者单位： | 北京医科大学第一医院普外科!100034 |
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| 摘 要： | 目的：探讨DCC基因在结直肠癌中杂合性缺失情况及其意义。方法：应用PCR技术检测了25例中国人散发型结直肠癌病人正常粘膜及癌组织和3个直肠癌细胞系DCC基因M2位点限制性片断长度多态性。结果：16例（64％）病人具有杂合性，其中4例（25％）癌组织表现出等位基因杂合性缺失。此4例均有淋巴结或肝转移。细胞系HCT，HR8348表现杂合性，HT29表现纯合性。结论：DCC基因是一个在结直肠肿瘤发生发展中起重要作用的抑癌基因，该基因的异常与结直肠癌发生发展有关。
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| 关 键 词： | 结肠肿瘤／遗传学 基因.DCC 基因缺失 聚合酶链反应 |
LOSS OF HETEROZYGOSITY INVOLV1NG THE DCC GENE IN HUMAN COLORECTAL CANCERS DEMONSTRATED BY THE POLYMERASE CHAIN REACTION |
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| XU Wenhuai, GAO Suikuan, WAN Yuanlian, LIU Zhanbing, LI Xiaowu, WANG Dongmin. LOSS OF HETEROZYGOSITY INVOLV1NG THE DCC GENE IN HUMAN COLORECTAL CANCERS DEMONSTRATED BY THE POLYMERASE CHAIN REACTION[J]. Journal of Peking University. Health sciences, 1997, 0(1) |
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| Authors: | XU Wenhuai GAO Suikuan WAN Yuanlian LIU Zhanbing LI Xiaowu WANG Dongmin |
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| Abstract: | To investigate the significance of allele loss of DCC (deleted in colorectal cancer)gene in Chinese colorectal cancer patients. Methods: Using PCR technique, we examined 25 colorectalcancer patients and three cell lines for loss of heterozygosity (LOH) of DCC gene about M2 polymorphicsite. Results: sixteen of the cases (64% ) were informative, and among them 4 cases (25% ) showed allelic deletion. All the 4 cases had lymphatic or hepatic metastasis. One cell line showed homozygosity.Conclusion: DCC gene is an important tumor suppressor gene, and its alterations play a role in thepathogenesis and progression of colorectal cancer. |
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| Keywords: | Colonic neoplasms/genet Genes DCC Gene deletion Polymerase chain reaction |
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