轮状病毒结构蛋白VP7 HLA-A2.1限制性CTL表位的初步鉴定

目的 预测并初步鉴定轮状病毒Wa株结构蛋白VP7 HLA—A2．1限制性CTL表位。方法 用BIMAS软件预测VP7 HLA—A2．1限制性CTL表位；分子模拟技术对分值最高的4条肽进行分子模建；最后测定候选肽与HLA-A2．1分子的亲和力及结合稳定性。结果 结合BIMAS及分子模拟的预测结果，选择4条肽QLYCDYNLV（132—140）、LLNYILKSV（18—26）、VLMKYDQSL（140—148）及VNWKKWWQV（287—295）作为候选表位肽；4条肽与HLA—A2．1结合的荧光系数（FI）值分别为2．58、3．83、3．19及0．82，肽-HLA-A2．1复合物半数解离时间（DC50）分别为2-4、6-8、8及2h。结论 QLYCDYNLV（132—140）、LLNYILKSV（18—26）及VLMKYDQSL（140—148）为潜在的HLA-A2．1限制性CTL表位。

Primary identification of HLA-A2.1-restricted CTL epitopes from rotavirus protein VP7

Objective To identify HLA-A2.1 restricted-CTL epitopes from protein VP7 of rotavirus strain Wa.Methods A compu-(ter)-based program BIMAS combined with computer-based molecular modeling were utilized to predict HLA-A2.1 restricted CTL epitopes within rotavirus protein VP7.T2 cells coated with peptides were used to determine the binding affinity and stability of HLA-A2.1-peptide complex.Results Four peptides with the highest score,which were QLYCDYNLV(132-140),LLNYILKSV(18-26),VLMKYDQSL(140-148) and VNWKKWWQV(287-295),were screened as candidate epitopes.The binding affinity of these peptides to HLA-A2.1 molecules,expressed as FI,were 2.58,3.83,3.19,and 0.82,respectively.The stability of HLA-A2.1-peptide complex was expressed as DC_(50),which were(2-4 h,) 6-8 h,8 h,and 2 h,respectively.Conclusion QLYCDYNLV(132-140),LLNYILKSV(18-26),and VLMKYDQ(SL(140-148)) might be HLA-A2.1-restricted CTL epitopes.