壳聚糖挂膜修饰聚丙烯网片与兔脂肪源性间充质细胞的黏附性研究

目的 研究壳聚糖挂膜修饰聚丙烯网片的技术参数及其与兔脂肪源性间充质细胞(ADSCs)的黏附性.方法 分别称取1、1.25和1.5g壳聚糖加2％的乙酸溶液、5％的戊二醛和50％的正庚烷溶解后,得到质量分数分别为2.0％、2.5％和3.0％的壳聚精铸膜液.将聚丙烯网片在壳聚糖铸膜液中反复浸泡、晾干后得到壳聚糖挂膜修饰的聚丙烯网片.将兔ADSCs接种于壳聚糖挂膜的聚丙烯网片上,培养24 h后观察细胞与材料的黏附性.结果 三种浓度的壳聚糖铸膜液挂膜修饰的聚丙烯网片柔软性均较挂膜前下降,壳聚糖铸膜液浓度越高,聚丙烯网片柔软性越低,而且3.0％铸膜液修饰的聚丙烯网片出现涂敷效果不均匀的现象.兔ADSCs在壳聚糖挂膜修饰的聚丙烯网片材料上生长良好.兔ADSCs与未挂膜的聚丙烯网片共培养24h后,表面未见细胞生长.结论 2.5％壳聚糖铸膜液为挂膜修饰聚丙烯网片的理想浓度.壳聚糖挂膜修饰的聚丙烯网片与兔ADSCs有良好的黏附性.

Adhesion behavior of rabbit adipose-derived stromal cells on chitosan-modified polypropylene mesh

Objective To study the technical parameters of chitosan-modified polypropylene mesh,and observe the adhesion behavior of rabbit adipose-derived stromal cells(ADSCs) on the modified mesh.Methods Chitosan of 1 g,1.25 g and 1.5 g were respectively dissolved into the mixture of 2% acetic acid solution,5% glutaraldehyde solution and 50% n-heptane solution,and 2.0%,2.5%,3.0% chitosan mould solutions were obtained.Polypropylene meshes were modified by different concentrations of chitosan mould solutions,and then the chitosan-modified polypropylene meshes were obtained.Rabbit ADSCs were cultured with chitosan-modified polypropylene meshes for 24 h,and the adhesion behavior of cells on materials was observed.Results Chitosan-modified polypropylene meshes were harder than non-modified polypropylene meshes,and the plasticity of polypropylene meshes decreased with the concentration increase of chitosan solutions.Polypropylene mesh modified by 3.0% chitosan solution had the phenomenon of uneven coating.After co-culture for 24 h,rabbit ADSCs grew well on modified polypropylene meshes,while there was no rabbit ADSCs growing on the non-modified polypropylene meshes.Conclusion The ideal concentration of the chitosan solution for modified polypropylene mesh is 2.5%,and chitosan-modified polypropylene mesh is suitable for rabbit ADSCs adhesion.