Identification of inflammatory cell phenotypes in human oral carcinomas by means of monoclonal antibodies |
| |
Authors: | Th Lning H Broemel WM Becker HF Otto |
| |
Institution: | Institute of Pathology (Director: Professor Dr G. Scifert), Martinistr, 52(UKE), D-2000, Hamburg 20, FRG |
| |
Abstract: | Monoclonal antibodies reacting with human T cell sub-populations, Langerhans cells and macrophages were used to examine the quantitative distribution of immune-competent cells in normal oral mucosa and invasive oral carcinomas. Both immunofluorescent and immunoperoxidase procedures were applied. In normal oral epithelia, the dominant immune-reactive cell was the Langerhans cell, positive for OKT 6 and expressing HLA-DR gene products (OKIal+). Many intra-epithelial non-epithelial cells (non-keratinocytes), belonged to the lymphocyte system carrying the suppressor/cytotoxic phenotype (OKT 8+). This lymphocyte sub-population was also the most prominent cell type in the normal mucosal stroma. The quantitative evaluation of immune-competent cells in squamous cell carcinomas revealed elevated numbers of all the inflammatory cell sub-populations investigated (suppressor/cytotoxic lymphocytes, helper/inducer lymphocytes, Langerhans cells, macrophages) compared with the normal oral mucosa. There was a striking increase in suppressor/cytotoxic lymphocytes (OKT 8+) and in cells of the macrophage system, including Langerhans cells (OKIal+, OKM l+, OKT 6+). In the stroma distant to the tumour complexes, many helper/inducer lymphocytes (OKT 4+) were also observed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|