| HPLC法测定苦碟子注射液中木犀草素的含量 |
| |
| 引用本文： | 陈富超,李鹏,方宝霞,陈茜,王亚文,狄书萌.HPLC法测定苦碟子注射液中木犀草素的含量[J].中国药师,2010,13(2):220-222. |
| |
| 作者姓名： | 陈富超 李鹏 方宝霞 陈茜 王亚文 狄书萌 |
| |
| 作者单位： | 1. 郧阳医学院附属东风医院药学部,湖北,十堰,442008
2. 郧阳医学院药检学院 |
| |
| 摘 要： | 目的：采用HPLC法测定苦碟子注射液中木犀草素的含量。方法：采用Kromasil—c18（250mm×4．6mm，5μm）色谱柱，乙腈-0．4％磷酸水溶液（25：75）为流动相，流速为0．8ml·min-1，柱温：30℃，检测波长为350nm。结果：木犀草素在1．1～13．2μg·ml-1浓度范围内线性关系良好（r=0．9998），平均加样回收率为98．76％，RSD为0．98％。结论：本方法简便准确，重复性好，可用于苦碟子注射液中木犀草素的含量测定。
|
| 关 键 词： | 木犀草素 苦碟子注射液 高效液相色谱法 |
Determination of Luteolin in Kudiezi Injection by HPLC |
| |
| Chen Fuchao,Li Peng,Fang Baoxia,Chen Xi,Wang Yawen,Di Shumeng.Determination of Luteolin in Kudiezi Injection by HPLC[J].China Pharmacist,2010,13(2):220-222. |
| |
| Authors: | Chen Fuchao Li Peng Fang Baoxia Chen Xi Wang Yawen Di Shumeng |
| |
| Institution: | ( Student of Yaojian College of Yunyang Medical College) |
| |
| Abstract: | Objective： To develop an HPLC method for the determination of luteolin in kudiezi injection. Method ： The Kromasil- Cls column （250 mm × 4. 6 mm,5 μm）was used, the mobile phase consisted of acetonitrile -0. 4% Phosphoric acid solution （25：75）, the flow rate was 0. 8 ml-min-1 ,the column temperature was 30 ℃ ,and the UV detector was set at 350 nm. Result： The cablibration curve was linear within the range of 1.1-13.2 μg.ml-1 ,the average recovery was 98. 76% and the RSD was 0.98%. Conclusion： The method is simple, accurate, and repeatable. It can be applied in determination of luteolin in kudiezi Injection. |
| |
| Keywords: | Luteolin Kudiezi Injection HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
