银杏叶提取物、卡托普利、缬沙坦单用及合用对高糖诱导的大鼠肾小球系膜细胞增殖肥大的影响

目的研究卡托普利、缬沙坦及中药银杏叶提取物（GBE）对高糖培养的大鼠肾小球系膜细胞增殖肥大的影响,探讨三者单用及合用时的异同。方法体外培养大鼠肾小球系膜细胞（MC）,分为正常（NS）组、甘露醇（MA）组、高糖（HG）组、溶媒（DMSO）组、GBE组、卡托普利（Cap）组、缬沙坦（Val）组以及GBE和卡托普利联合用药（GC）组、GBE和缬沙坦联合用药（GV）组。流式细胞仪测定细胞周期,MTT法检测细胞增殖变化情况。结果对高糖引起的细胞肥大,GBE单用、GBE联合Cap、GBE联合Val均可有效逆转,而Cap和Val单用时无此作用;对高糖诱导的细胞增殖,Cap、Val单用以及GBE联合Cap、GBE联合Val均可使其逆转,但GBE对其无明显影响。结论银杏叶提取物和卡托普利、缬沙坦联合应用时对大鼠MC的细胞保护作用更优。

Effects of monotherapy and combination therapy of Ginkgo biloba extract,captopril, valsartan on high glucose-induced proliferation and hypertrophy of rat mesangial cells

Objective To investigate the effect of captopril（CAP）,valsartan（VAL） and Chinese crude drug Ginkgo biloba extract（GBE） on rat glomerular mesangial cells（MC） cultured in high glucose and to explore the differences between monotherapy and combination therapy of the drugs so as to provide rationale for captopril or valsartan combination therapy.Methods The rat MC line HBZY-1 was cultured in vitro in DMEM media.The cultured cells were divided into normal standard group（NS group,final concentration of 5.56 mmol·L-1 glucose）,mannitol group（MA group,5.56 mmol·L-1 glucose ＋ 24.44 mmol·L-1 mannitol）,high glucose group（HG group,final concentration of 30 mmol·L-1 glucose）,solvent control group（DMSO group,30 mmol·L-1 glucose ＋ 0.1% DMSO）,Ginkgo biloba extract group（GBE group,30 mmol·L-1 glucose ＋ 0.1 % DMSO＋20 g·L-1 GBE）,captopril group（Cap group,30 mmol·L-1 glucose ＋ 0.1 % DMSO＋1 μmol·L-1 captopril）,valsartan group（Val group,30 mmol·L-1 glucose ＋ 0.1 % DMSO ＋ 10 μmol·L-1 valsartan）,captopril-GBE combination therapy group（GC group,30 mmol·L-1 glucose ＋ 0.1 % DMSO ＋ 20 g·L-1 GBE ＋ 1 μmol·L-1 captopril）,and valsartan-GBE combination therapy group（GV group,30 mmol·L-1 glucose ＋ 0.1 % DMSO ＋ 20 g·L-1 GBE ＋ 10 μmol·L-1 valsartan）.After corresponding treatment,the cell cycles were determined by flow cytometry,and cell proliferation was evaluated by MTT.Results GBE and its combination with either captopril or valsartan could reverse cell hypertrophy induced by high glucose,while neither captopril nor valsartan monotherapy could achieve that effect.Likewise,GBE and its combination with either captopril or valsartan could reverse cell proliferation induced by high glucose,while GBE had no significant effect.Conclusions GBE in combination with either captopril or valsartan had better cytoprotective effect on rat MC.