HPLC-PDA法测定速效止泻胶囊中盐酸小檗碱的含量

目的建立以高效液相色谱法测定速效止泻胶囊中盐酸小檗碱和没食子酸含量的方法。方法采用SunfireTM C18(5μm,4.6 mm×150 mm)色谱柱,柱温:35℃。流动相为甲醇-乙腈-0.1%磷酸(3:2:95),采用梯度洗脱程序,流速1.0 ml·min-1,进样量为20μl,检测波长:270 nm。结果盐酸小檗碱在20.931~209.31 mg.L-1范围内呈良好的线性关系,r=1.000 0,平均回收率为100.02%,RSD=1.5%(n=6)。没食子酸在13.075~130.754 mg.L-1范围内呈良好的线性关系,r=1.000 0。结论该方法简单、快速、准确、重现性好,能够较好地控制该产品的质量。

Determination of berberine hydrochloride and gallic acid in Suxiao Zhixie capsules by HPLC

Aim To establish a method for the determination of berberine hydrochloride and gallic acid in Suxiao Zhixie capsules.Methods The separation was performed on SunfireTM C18column（5 μm,4.6 mm×150 mm）.The column temperature was 35℃.The mobile phase was methonal-acetonitrile-0.1%phasphoric acid（3295）and eluted with a gradient.The flow rate was 1.0 ml·min-1.The injection volume was 20 μl,The detection wavelength was at 270 nm.Results The linear of berberine hydrochloride was in the range of 20.931~209.31 mg·L-1（r=1.000 0）.The average recovery was 100.02% and RSD was 1.5%（n=6）.The linear of gallic acid was in the range of 13.075~130.754 μg·L-1（r=1.000 0）.Conclusions The method is convenient,rapid,economical and accurate.It can be used for quality control of Suxiao Zhixie capsules.