RP-HPLC同时测定附子地上部分中新乌头碱和次乌头碱的含量

目的 建立RP-HPLC法,测定附子地上部分中新乌头碱和次乌头碱的含量.方法 采用Venusil ABS C18色谱柱(250 mm × 4.6 mm,5 μm),以乙腈-0.1% 乙二胺溶液为流动相,梯度洗脱,柱温30 ℃,流速 1 ml·min-1,检测波长230 nm.结果 新乌头碱、次乌头碱均得到较好分离,分别...

Determination of mesaconitine and hypaconitine in the aerial part of Radix Aconiti Lateralis Praeparata by RP-HPLC

Aim To develop an optimal RP-HPLC method for determining the content of mesaconitine and hypaconitine in the aerial part of Radix Aconiti Lateralis Praeparata.Methods Two constituents were analyzed simultaneously with a Venusil ABS C18 column（250 mm × 4.6 mm,5 μm） by gradient elution using acetonitrile-0.1% ethylenediamine as the mobile phase.The column was maintained at 30 ℃.The flow rate was 1 ml·min-1.The detection wavelength was set at 230 nm.Results Excellent chromatographic separation was achieved for mesaconitine and hypaconitine.The linear ranges of the two constituents were 12.5~1 200 mg·L-1,and 11.46~1 100 mg·L-1 respectivety.The extraction recoveries were 99.72%（RSD=0.69%）,and 100.29%（RSD=1.07%）.ConclusionThe method has the advantages of simplicity,acuteness and precision,which is suitable for quantitative analysis of mesaconitine,hypaconitine in Radix Aconiti Lateralis Praeparata.