N-硝基-L-精氨酸甲酯对实验性大鼠隐睾Bcl-2表达的影响

①目的研究一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯对实验性大鼠隐睾生精细胞凋亡过程中B细胞淋巴瘤/白血病-2（Bcl-2）表达的影响。②方法 42天龄雄性SD大鼠建立单侧隐睾模型,随机分为隐睾组、隐睾＋L-NAME组、隐睾＋生理盐水（溶媒）组、假手术组,每组12只大鼠,隐睾＋L-NAME组和隐睾＋生理盐水组大鼠术后分别腹腔内注射L-NAME或生理盐水,每天1次,定时定量（50mg/Kg）,术后第7天分别采用RT-PCR和免疫组织化学方法检测各组隐睾Bcl-2mRNA和蛋白表达的变化。③结果与假手术组睾丸相比,隐睾组、隐睾＋生理盐水组睾丸生精上皮和睾丸间质细胞内Bcl-2mRNA和蛋白表达降低,差异有显著性（P〈0.05）;而隐睾＋L-NAME组Bcl-2 mRNA和蛋白与假手术组睾丸无明显差别。④结论 L-NAME可通过调节Bcl-2的表达抑制热应激导致的生精细胞凋亡。

Effect of L-NAME on the expression of Bcl-2 in the rat cryptorchidism

Objective To investigate the effect of L-nitroarginine methyl ester（L-NAME） on the expression of Bcl-2 in apoptosis of spermatogenic cells in the rat cryptorchidism.Methods Unilateral cryptorchidism model was surgically induced in the 42-day old rats and randomly divided into cryptorchidism model group,cryptorchidism group＋ L-NAME group,cryptorchidism＋ saline group and sham operation group（each group 12 cases）.Except for the sham operation group and cryptorchidism group,the rats were injected intraperitoneally normal saline and L-NAME50mg/（Kg·d） respectively.The mRNA and protein expression of Bcl-2 in the rat cryptorchidism was detected by RT-PCR and immunohistochemical SABC assays on the 7th day after the operation.Results Compared to the sham operation group,the mRNA and protein expression of Bcl-2 in spermatogenic epithelium and Leydig cells decreased significantly in the cryptorchidism group and cryptorchid ＋ normal saline group（P〈0.05）,while there was no significant difference between the cryptorchid testes treated with L-NAME and the sham operation group.Conclusion L-NAME could inhibit spermatogenic cell apoptosis by promoting Bcl-2 expression in rats experimental cryptorchidism.