| Abstract: | It is known that intracellular Ca2+ is overloaded during
ischemia,and at the same tirne,the activity of one of the major
pathways of ca2+ efluxs-Na+/Ca2+ exchanger is altered.However,the
molecular mechanism of this alteration is still unknown. We cloned a
456bp cDNA fragment of rat cerebellar Na+/Ca2+ exchanger gene.Using
this fragment as the probe,We found that the Na+ /Ca2+ exchanger mRNA
is widely distributed in rat central and peripheral nervous
system,such as in cerebra, heart,lung, and kidney. Using focal
cerebral ischemic model,We de-tected the gene expression of Na+/Ca2+
exchanger in ischemic brain by Northern blotting and in situ
hybridization,and found that in ischemic tissues,the mRNA level of
Na+/Ca2+ exchanger is lowered. |
