前列活血汤对自身免疫性前列腺炎大鼠模型前列腺内炎性细胞因子的影响

目的观察前列活血汤对自身免疫性前列腺炎大鼠模型(EAPAM)前列腺组织内炎性细胞因子的影响,从而探讨中药治疗前列腺炎的作用机制和作用靶点。方法60只雄性Wistar大鼠选择40只,随机分成四组:正常对照组、模型组、前列泰组、前列活血汤组,每组10只;余20只制作前列腺组织抗原用。除正常组外,余三组经腹部皮下多点注射大鼠前列腺组织抗原造模(EAPAM)。免疫造模3天后,除正常组外,余三组动物分别予生理盐水、前列泰、前列活血汤灌胃8周。动物处死后光镜下观察各组大鼠前列腺组织的病理改变;酶联免疫吸附法(ELISA)检测大鼠前列腺组织中白介素8(IL-8)、白介素10(IL-10)、肿瘤坏死因子α(TNF-α)的水平;聚合酶链式反应法(PCR)检测大鼠前列腺组织中TNF-αmRNA的表达水平。结果模型组前列腺组织中IL-8、IL-10、TNF-α、TNF-αmRNA表达水平较正常对照组均明显升高(P0.05);前列腺活血汤组前列腺组织中IL-8、IL-10、TNF-α、TNF-αmRNA表达水平较模型组均有所降低(P0.05)。结论前列活血汤能通过调节前列腺局部免疫状态从而明显减轻模型大鼠前列腺的炎症反应。

Effect of Qianliehuoxue Decoction on Inflammatory Factors in Rat Model of Autoimmune Prostatitis

Objective To explore the impacts of qianliehuoxue decoction on inflammatory factors in prostate tissue in rat model with autoimmune prostatitis（EAPAM）.Methods 40 rats were selected from 60 male Wistar rats and were randomized into 4 group,named normal control group,model group,qianlietai tablet group and qianliehuoxue decoction group,10 rats in each group.The rest 20 rats were for the preparation of prostate tissue antigen.Except the rats in normal group,the rats in the other three groups were injected peritoneally subcutaneously in the abdomen on multiple sites with prostate tissue antigen（EAPAM）for modeling.3 days after immune modeling,except the rats in normal group,the rats in the rest three groups were administered with gastric perfusion with normal saline,qianlietai and qianliehuoxue decoction separately for 8 weeks.After sacrificed,the pathologic changes of prostatic tissue were observed under optical microscope in each group.The levels of interleukin 8（IL-8）,interleukin 10（IL-10）and tumor necrosis factor α（TNF-α）in prostatic tissue were detected by enzyme linked immunosorbent assay（ELISA）.The expression level of TNF-α mRNA in prostatic tissue was detected by polymerase chain reaction（PCR）.Results Compared with normal group,the expression levels of IL-8,IL-10,TNF-α and TNF-α mRNA in prostate tissue of model group increased apparently（P〈0.05）.The expression levels of IL-8,IL-10,TNF-α and TNF-α mRNA in prostate tissue of qieliehuoxue decoction group decreased as compared with normal group（P〈0.05）.Conclusion Qieliehuoxue decoction can alleviate apparently inflammatory reaction of model rat prostate gland by regulating local prostate immune condition.