| 高效液相色谱法测定参菊洗剂中苦参碱的含量 |
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| 引用本文： | 张广求,王树平,刘文,田祥学,谭璐.高效液相色谱法测定参菊洗剂中苦参碱的含量[J].中国医院用药评价与分析,2014(3):232-234. |
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| 作者姓名： | 张广求 王树平 刘文 田祥学 谭璐 |
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| 作者单位： | [1]湖北省黄冈市中心医院药剂科,湖北黄冈438000 [2]湖北省黄冈市食品药品监督检验中心,湖北黄冈438000 |
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| 基金项目： | 黄冈市科学技术局2013年度市级科技计划项目（编号：黄科字[2013]76号） |
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| 摘 要： | 目的：建立测定参菊洗剂中苦参碱含量的方法.方法：采用高效液相色谱法.色谱柱为DionexC18（4.6mm×250.0mm,5μm）;流动相为乙腈-水-三乙胺（65∶35∶0.2）;流速为1.0ml/min,检测波长为220nm,柱温为30℃.结果：苦参碱在39.64～356.76μg/ml浓度范围内呈良好的线性关系（r=0.9999,n=6）,平均回收率为99.26％,RSD为0.87％（n=9）.结论：本文建立的方法简便、准确、可靠,专属性强,适用于参菊洗剂中苦参碱的常规分析和质量控制.
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| 关 键 词： | 高效液相色谱法参菊洗剂苦参碱含量测定 |
DeterminationofMatrineinShenjuLotionbyHPLC |
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| ZHANG Guang-qiu WANG Shu-ping LIUWen TIAN Xlang-xue,TAN Lu.DeterminationofMatrineinShenjuLotionbyHPLC[J].Evaluation and Analysis of Drug-Use in Hospital of China,2014(3):232-234. |
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| Authors: | ZHANG Guang-qiu WANG Shu-ping LIUWen TIAN Xlang-xue TAN Lu |
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| Institution: | 1. Dept. of Pharmacy, Hubei Huanggang Municipal Central Hospital, Hubei Huanggang 438000, China; 2. Hubei Huanggang Center for Food and Drug Control, Hubei Huanggang 438000, China) |
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| Abstract: | OBJECTIVE：To establish a method for determination of the content of matrine in Shenju lotion. METHODS：The content of matrine in Shenju lotion was determined by HPLLC using Dionex Cls column（4.6 mm x 250.0 mm, 5 μm） with the mobile phase consisting of acetonitrile-water-triethylamine （ 65 ： 35 ： 0. 2 ） at flow rate of 1 ml/min,detective wavelength of 220 nm and the column temperature of 30 ℃. RESULTS：The linear range of matrine was 39.64-35 6.76 μg/ml（r =0. 999 9,n =6）. The average recovery was 99.26% and the relative standard deviations was 0. 87% （n = 9）. CONCLUSION：The established method is simple, accurate, reliable and specific and it can be used for routine analysis and quality control of matrine in Shenju lotion. |
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| Keywords: | HPLC Shenju lotion Matrine Determination |
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