| pcDNA3-hBMP2转染兔骨髓基质细胞前后生长特性的研究 |
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| 引用本文： | 魏利成,刘丹平,蒲勤,王朝燕,蒋清,尹建文,张正. pcDNA3-hBMP2转染兔骨髓基质细胞前后生长特性的研究[J]. 中国骨与关节损伤杂志, 2009, 24(1): 52-54 |
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| 作者姓名： | 魏利成 刘丹平 蒲勤 王朝燕 蒋清 尹建文 张正 |
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| 作者单位： | 湖南省邵阳市第一人民医院骨科,422000;锦州医学院附属第一医院骨科;第四军医大字生化教研室 |
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| 基金项目： | 国家自然基金资助项目(30672125) |
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| 摘 要： | 目的 观察pcDNA3-hBMP2转染兔骨髓基质细胞前后的生长特性变化,为骨组织工程的种子细胞选择打下基础.方法 抽取成年雄性新西兰大白兔髂骨骨髓,密度梯度离心获得骨髓基质细胞,培养传至第5代,按处理方法分为常规培养液组(A组),pcDNA3-hBMP2转染组(B组).观察原代MSC及第5代的细胞形态等生长特性并与转染组进行比较.结果 原代MSCs为短梭形,簇形生长,传代细胞呈长梭形,旋涡形生长.转染组细胞72 h后表达BMP2,瞬问表达为100%.转染细胞未经筛选培养4周细胞形态发生较明显地变化,细胞生长周期改变.结论 pcDNA3-hBMP2成功转染兔骨髓基质细胞并改变其生物学特性.
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| 关 键 词： | 组织工程 骨髓基质细胞 生长特性 |
Comparative Study on Main Biological Characteristics of Pre-transfected Rabbit Marrow Stromal Cells and Post-transfected with pcDNA3-hBMP2 |
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| Wei Licheng,Liu Danping,Pu Qin,et al.. Comparative Study on Main Biological Characteristics of Pre-transfected Rabbit Marrow Stromal Cells and Post-transfected with pcDNA3-hBMP2[J]. Chinese Journal of Bone and Joint Injury, 2009, 24(1): 52-54 |
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| Authors: | Wei Licheng Liu Danping Pu Qin et al. |
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| Affiliation: | Wei Licheng,Liu Danping,Pu Qin,et al.Department of Orthopedics,the First People Hospital,Shaoyang,422000 |
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| Abstract: | Objective To observe main biological characteristic and changes before and after MSCs were transfected with pcDNA3-hBMP2.Methods MSCs from an adult New Zealand white rabbit were isolated and cultivated.MSCs were divided into two groups.Group A was transfected with pcDNA3-hBMP2 and group B was not transfected as control group.Immunohistochemical staining was used for detecting BMP2 expressing 2 days after transfection.The cell morphological changes,cell divide index and cell cycle were observed after transfe... |
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| Keywords: | Tissue engineering Bone marrow stromal cells Biological characteristics |
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