| 活细胞工作站技术在小鼠卵母细胞实时观察中的应用 |
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| 引用本文： | 毕晔,张克梅.活细胞工作站技术在小鼠卵母细胞实时观察中的应用[J].临床检验杂志,2011,29(9):678-679. |
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| 作者姓名： | 毕晔 张克梅 |
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| 作者单位： | 南京医科大学组织胚胎学系，南京210029;南京医科大学组织胚胎学系，南京210029 |
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| 摘 要： | 摘要：目的：将活细胞工作站（LCS）应用于实时观察小鼠卵母细胞后期成熟和极体排放过程。 方法：运用LCS技术平台，利用hoechst 33342标记DNA，同时结合明场观察，实时记录小鼠卵母细胞后期成熟和第二极体排放过程。 结果：在建立最适工作条件后，成功将LCS技术平台应用于小鼠卵母细胞观察，获得了第二极体排放的实时影像。 结论：LCS技术可成功地应用于小鼠卵母细胞观察，为进一步扩展LCS应用范围，获得卵母细胞及早期胚胎生长发育过程的实时影像打下良好的基础。
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| 关 键 词： | 活细胞工作站 卵母细胞 极体排放 |
| 收稿时间： | 2011/5/24 0:00:00 |
| 修稿时间： | 2011/8/20 0:00:00 |
Application of live cell imaging system in real time observation of mouse oocyte development |
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| BI Ye,ZHANG Ke-mei.Application of live cell imaging system in real time observation of mouse oocyte development[J].Chinese Journal of Clinical Laboratory Science,2011,29(9):678-679. |
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| Authors: | BI Ye ZHANG Ke-mei |
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| Institution: | BI Ye,ZHANG Ke-mei(Department of Histology and Embryology,Nanjing Medical University,Nanjing 210029,Jiangsu,China) |
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| Abstract: | Objective To establish a platform of live cell imaging system(LCS) applied in real-time observation of late maturation and emission process of the second polar body in mouse oocytes in vitro.Methods By using LCS platform,the late maturation and the second polar body emission process of mouse oocytes were observed and recorded in real-time by staining with DNA dye hoechst 33342 and bright field.Result By a series of screening,the live cell imaging platform was established successfully and applied in observat... |
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| Keywords: | live cell station oocyte polar body emission |
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