灯盏花素对实验性肺纤维化大鼠的干预作用

目的探讨灯盏花素对博莱霉素致肺纤维化大鼠肺组织细胞凋亡、Fas/FasL表达以及氧自由基的影响。方法48只大鼠随机分为对照组(16只)、模型组(16只)以及灯盏花素组(16只),气管内注入博莱霉素复制大鼠肺纤维化模型,次日灯盏花素组每日腹腔内注射灯盏花素注射液10mg.kg-1,于d7及d28每组分别处死8只大鼠,运用流式细胞仪测定肺纤维化大鼠肺组织细胞凋亡率、Fas/FasL表达,用TAB法测定肺组织匀浆丙二醛含量以及肺组织匀浆羟脯氨酸含量。结果炎症期和纤维化期大鼠肺组织细胞凋亡率增加,Fas/FasL表达增多,丙二醛及羟脯氨酸含量增高(P<0.01),而灯盏花素能明显降低肺组织的凋亡率,降低丙二醛及羟脯氨酸含量,下调Fas/Fasl的阳性表达。结论灯盏花素能减慢肺间质纤维化的进程,对肺间质纤维化有一定的保护作用。

The effect of breviscapine on pulmonary fibrosis induced by bleomycin in rats

Aim To investigate the expressions of Fas/FasL and apoptosis of pulmonary tissue as well as oxygen free radical in the pulmonary fibrosis induced by bleomycin in rats at inflammation stage and fibrosis stage.Methods Forty-eight rats were divided into three groups randomly,including control group,model group and breviscapine group.On experimental day 0,the rats were intratracheally instilled with bleomycin,and then treated with breviscapine 10 mg·kg-1·d-1 until they were killed.On days 7,28 after stillation,eight rats of each group were killed and the lungs were harvested for examination.Flow cytomagnetometry was used to detect the changes of cell apoptosis and the expressions of Fas/Fasl in rats with pulmonary fibrosis caused by bleomycin at flammation stage and fibrosis stage.Malonaldehyde and hydroxyproline(HYP)of lung tissue were detected by TAB method.Results The apoptosis index of lung cells in the pulmonary fibrosis group was higher than that of control and breviscapine group.The expression of Fas/FasL and contents of Malonaldehyde as well as hydroxyproline in the pulmonary fibrosis group were higher than those of control group and breviscapine group(P<0.01).Conclusion Breviscapine may alleviate the process of rat pulmonary fibrosis induced by bleomycin,and has protective effect on rats with pulmonary fibrosis.