| 乳腺癌细胞通过PTHrP调节成骨细胞MCP-1基因表达 |
| |
| 引用本文： | 张珊珊,李晓霞,王宝利.乳腺癌细胞通过PTHrP调节成骨细胞MCP-1基因表达[J].天津医药,2010,38(2). |
| |
| 作者姓名： | 张珊珊 李晓霞 王宝利 |
| |
| 作者单位： | 1. 天津医科大学代谢病医院内分泌研究所、卫生部激素与发育重点实验室,300070
2. 天津医科大学基础医学院微生物学教研室 |
| |
| 基金项目： | 天津市自然科学基金重点项目，天津市高等学校科技发展基金项目 |
| |
| 摘 要： | 目的:探讨乳腺癌细胞分泌的甲状旁腺激素相关蛋白(PTHrP)对成骨细胞单核细胞趋化蛋白-1(MCP-1)基因表达的调节作用.方法:首先将10~(-8)mol/L剂量的PTHrP(1-34)作用于成骨细胞UMR106,同时设立对照组,半定量RT-PCR法分析对不同时间成骨细胞的MCP-1 mRNA表达;之后构建PTHrP受体(PTH1R)的shRNA重组质粒PrH1R/pRNAT,将其转染至成骨细胞UMR106,建立稳定表达的细胞系PTH1R/UMR106;将乳腺癌细胞MDA-MB231条件培养基(CM)分别作用于UMR106和PTH1R/UMR106,半定量RT-PCR法测定MCP-1的mRNA表达.结果:PTHrP可以促进成骨细胞MCP-1的表达,起始于3 h,6 h到达高峰(P＜0.05).乳腺癌细胞CM作用UMR106成骨细胞6 h后,MCP-1表达水平也较未处理组明显升高(分别为1.238 1±0.115 5和0.5984±0.0364,P＜0.05),而采用RNA干扰技术敲减PrrH1R表达后,乳腺癌细胞CM对成骨细胞MCP-1表达的作用明显减弱(降低至0.867 2±0.045 7,P＜O.05).结论:乳腺癌细胞可通过PTHrP调节成骨细胞MCP-1.
|
| 关 键 词： | 乳腺肿瘤 癌 甲状旁腺素 蛋白质类 成骨细胞 单核细胞化单吸引蛋白质类 |
Regulation of MCP-1 in Osteoblasts by PTHrP in Breast Cancer Cells |
| |
| ZHANG Shanshan,LI Xiaoxia,WANG Baoli.Regulation of MCP-1 in Osteoblasts by PTHrP in Breast Cancer Cells[J].Tianjin Medical Journal,2010,38(2). |
| |
| Authors: | ZHANG Shanshan LI Xiaoxia WANG Baoli |
| |
| Institution: | ZHANG Shanshan,LI Xiaoxia,WANG Baoli Institute of Endocrinology,Metabolic Disease Hospital,Tianjin Medical University,Tianjin 300070,China |
| |
| Abstract: | Objective:To identify the regulation of monocyte chemoattractant protein-1 (MCP-1) in osteoblasts by parathyroid hormone related protein(PTHrP) in breast cancer cells.Methods:Osteoblast-like UMR106 cells were treated with 10-8 mol/L PTHrP (1-34) for different times and the expression of MCP-1 was studied by semiquantitative RT-PCR.Recombinant shRNA plsmid PTH1R/pRNAT was constructed and transfected into UMR106 cells.Stable PTH1R/UMR106 cells were established,in which PTH1R expression was knocked-down.The co... |
| |
| Keywords: | breast neoplasms carcinoma pathyroid hormone proteins osteoblasts monocyte chemoattractantproteins |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
