Ropinirole alters gene expression profiles in SH-SY5Y cells: a whole
genome microarray study |
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| Authors: | M.Z. Zhu W.D. Le G. Jin |
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| Affiliation: | 1.School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai, China;2.Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine, Shanghai, China;3.Department of Neurology, Baylor College of Medicine, Houston, TX., USA;4.ShanghaiBio Corporation, North Brunswick, NJ, USA;5.Shanghai Biochip Co., Ltd and National Engineering Center for Biochip at Shanghai, Shanghai, China |
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| Abstract: | Ropinirole (ROP) is a dopamine agonist that has been used as therapy for Parkinson''sdisease. In the present study, we aimed to detect whether gene expression wasmodulated by ROP in SH-SY5Y cells. SH-SY5Y cell lines were treated with 10 µM ROP for2 h, after which total RNA was extracted for whole genome analysis. Gene expressionprofiling revealed that 113 genes were differentially expressed after ROP treatmentcompared with control cells. Further pathway analysis revealed modulation of thephosphatidylinositol 3-kinase (PI3K) signaling pathway, with prominent upregulationof PIK3C2B. Moreover, batches of regulated genes, includingPIK3C2B, were found to be located on chromosome 1. These findingswere validated by quantitative RT-PCR and Western blot analysis. Our study,therefore, revealed that ROP altered gene expression in SH-SY5Y cells, and futureinvestigation of PIK3C2B and other loci on chromosome 1 may providelong-term implications for identifying novel target genes of Parkinson''s disease. |
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| Keywords: | Parkinson''s disease Gene expression Ropinirole SH-SY5Y |
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