Major histocompatibility complex (MHC) antigens polymorphism and alloimmunization study in thalassemia patients with febrile non-hemolytic transfusion reaction (FNHTR) |
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| Affiliation: | 1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran;2. Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran;1. Department of Transfusion Medicine, Homi Bhabha Cancer Hospital, Tata Memorial Centre, Sangrur, Punjab, India;2. Department of Immunohematology and Blood Transfusion, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand, India;3. Department of Transfusion Medicine, Shrimann Super Specialty Hospital, Jalandhar, Punjab, India;1. Department of Internal Medicine, Spaarne Gasthuis, Haarlem/Hoofddorp, The Netherlands;2. Department of Internal Medicine, Amsterdam UMC, Location AMC, The Netherlands;3. Department of Internal Medicine, Albert Schweitzer Hospital, Dordrecht, The Netherlands;4. Department of Hematology, Haga Teaching Hospital, The Hague, The Netherlands;5. Department of Internal Medicine, Jeroen Bosch Hospital, Den Bosch, The Netherlands;6. Department of Hematology, Amsterdam UMC, location VU University Medical Center, Amsterdam, The Netherlands;1. Laboratoire de Biologie Clinique, Faculté des Sciences Pharmaceutiques, Université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo;2. Centre d’Excellence et d’Expertise des hépatites virales et autres pathologies, Lubumbashi, Democratic Republic of the Congo;3. Faculté de Médecine, Université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo;4. Laboratoire de Microbiologie Expérimentale et Pharmaceutique, Faculté des Sciences Pharmaceutiques, Université de Kinshasa, Kinshasa, Democratic Republic of the Congo;5. Institut de Recherche Expérimentale et Clinique, Pôle de Microbiologie, Université Catholique de Louvain, Brussels, Belgium;1. Lancaster Medical School, Lancaster University, Lancaster, United Kingdom;2. Imperial College London;3. Anthony Nolan, London, United Kingdom;1. National Center for Disease Control and Public Health of Georgia, Tbilisi, Georgia;2. The University of Georgia, School of Health Sciences, Tbilisi, Georgia;3. Johns Hopkins University School of Medicine, Baltimore, United States;4. Division of Viral Hepatitis, National Center for HIV, Viral Hepatitis, STD and TB Prevention, CDC, Atlanta, United States;5. The Task Force for Global Health, Tbilisi, Georgia |
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| Abstract: | ObjectivesHLA alloimmunization is one of the most troublesome consequence of regular transfusion which is itself a mainstay measure to provide longevity to the thalassemia patients. Febrile non-hemolytic transfusion reaction (FNHTR) is one of the most common complication which might be related to the HLA alloimmunization. Here, we studied the HLA antigenic system and alloimmunization rate in the Iranian β-thalassemia patients who suffered from FNHTR compare to the β-thalassemia patients without FNHTR.Materials & methodsTotal of 60 β-thalassemia patients with FNHTR (case group) and 20 β-thalassemia patients without FNHTR (control group) randomly have been selected and enrolled in the study. All were tested for HLA-A and -B loci by PCR-SSP method and also for the presence of anti-lymphocyte antibodies by LIFT method. Comparisons between two groups were performed by Pearsonʼs χ2 test.ResultsTotally, a significant predominance was noted for two HLA alleles, HLA-A*24 (P = 0.029) and B*55 (P = 0.034) which have higher prevalence in control group. Although no significant association was found between the presence of anti-leukocyte antibodies and the development of FNHTR, the HLA-A*32 (P = 0.047) allele was considered as possible genetic markers in the susceptibility to the development of anti-leukocyte antibodies.ConclusionHere some evidences about the possible role of HLA polymorphism in susceptibility to FNHTR are provided. Those results indicated that HLA-A*24 and HLA-B*55 might play protective role on inducing FNHTR in β-thalassemia patients. Further studies which investigate the allele level of HLA-I alongside with specific reactivity of HLA-I antibodies might reveal more deep data about these phenomena. |
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| Keywords: | Human Leukocyte Antigens (HLAs) Febrile non-hemolytic transfusion reactions (FNHTR) Alloimmunization β-thalassemia Lymphocyte immunofluorescence test (LIFT) |
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