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Regeneration processes of alveolar bone and microvascular changes after the application of platelet-rich fibrin |
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| Institution: | 1. Department of Environmental Pathology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa, 238-8580, Japan;2. Department of Clinical Oral Anatomy, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa, 238-8580, Japan;1. Head, Dental Department, The Military University Hospital Prague, Prague, Czech Republic; and PhD student, 1st Medical Faculty, Charles University, Prague, Czech Republic;2. Development Director, Private practice, Prague, Czech Republic;3. Head, Technical Department, Private practice, Prague, Czech Republic;4. Professor, Department of Dentistry, 2nd Medical Faculty and Faculty Hospital Motol, Charles University, Prague, Czech Republic;1. Oral Pathology, Oral Pathology Department, Faculty of Dentistry, Tanta University, Egypt;2. Biology and Head of Confirmatory Diagnostic Unit, VACSERA Egypt;3. Pathology, Pathology Department, Faculty of Medicine, Cairo University, Egypt;1. Laboratory of Veterinary Anatomy, Nippon Veterinary and Animal Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo, 180–8602, Japan;2. Department of Oral Anatomy and Developmental Biology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan;1. Department of Basic and Clinical Oral Sciences, Faculty of Dental Medicine, Umm Al-Qura University, Saudi Arabia;2. Department of Oral Biology, Faculty of Dentistry, Alexandra University, Egypt;1. Department of Pharmacology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan;2. Department of Pharmacology, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan |
| Abstract: | ObjectivesPlatelet-rich fibrin (PRF) is a promising agent for bone regeneration (BR). Platelets contain several growth factors that promote angiogenesis and BR. In this study, we observed the morphology of alveolar BR.MethodsPRF (Advanced PRF: A-PRF) was prepared by extracting 10 mL of blood from each dog in a collection tube before tooth extraction. The samples were centrifuged at 200 × g for 8 min and incubated for 10 min to allow clotting. The alveolar socket on the dentition's right side was densely filled with PRF. The opposite side, which did not receive PRF, served as the control group. Different methods were used for specimen preparation and observation. Sections stained with hematoxylin and eosin were observed under a light microscope. Bone specimens were observed using stereoscopic microscopy. The resin cast models were examined using a scanning electron microscope. Moreover, bone formation ratio and height were measured.ResultsFourteen days postoperatively, angiogenesis and bone deposition were more advanced in the PRF group than in the control group. Thirty days postoperatively, both groups developed porous bone. In the PRF group, new bone trabeculae (BT) and a network of blood vessels were formed in the bone marrow. Ninety days postoperatively, the resin cast showed a normal bone structure with BT and bone marrow. Thick BT were observed in the PRF group.ConclusionsGrowth factors in PRF stimulate microcirculation and promote angiogenesis and bone deposition. The benefits of PRF include safety and increased bone formation. |
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