干扰素调节因子1、3、7在原发性舍格伦综合征中的表达

目的：检测原发性舍格伦综合征（primary Sjgren＇s syndrome,pSS）患者血细胞中干扰素调节因子（IRF）1、3、7的表达水平,并检测IRF1在pSS患者腮腺组织中的表达。方法：采用实时定量PCR,对37例pSS患者和24例对照组患者血细胞中IRF1、3、7的水平进行检测,比较分析其在两组之间的差异。采用免疫组化和免疫荧光法检测IRF1在pSS患者腮腺组织中的表达。应用SAS6.12软件包对数据进行t检验。结果：实时定量PCR法检测结果发现,pSS组的IRF1mRNA表达量显著高于对照组,两组之间有显著差异（P=0.00001）,pSS组的IRF1是对照组的2.17倍,而IRF3和IRF7在两组之间无显著差异（P=0.3813,0.4501）。免疫组化和免疫荧光发现,pSS组患者腮腺组织中的导管上皮细胞、淋巴细胞和上皮岛均为IRF1阳性;而对照组腮腺组织中仅导管上皮细胞为IRF1阳性。结论：IRF1在原发性舍格伦综合征患者中存在异常表达。

Expression of interferon regulatory factor 1,3,and 7 in primary Sj(o)gren's syndrome

PURPOSE： To investigate the level of interferon regulatory factor （IRF） 1,3 and 7 in peripheral blood cells from patients with primary Sjgren’s syndrome （pSS） and determine whether and where IRF1 exists in the parotid glands of pSS.METHODS： Peripheral blood cells and parotid gland biopsy specimens from patients with pSS were studied.IRF1,IRF3 and IRF7 gene mRNA levels in peripheral blood cells were calculated by using real-time PCR.The IRF1 positive cells in the parotid glands with pSS were observed by using immunohistochemistry and immunofluorescence.Statistical analysis was performed by Student’s t test using SAS 6.12 software package.RESULTS： Compared with 24 controls,the IRF1 mRNA levels in peripheral blood cells of 37 cases with pSS were up-regulated （P0.05）,but the IRF3 and IRF7 mRNA levels of pSS weren’t up-regulated （P0.05）.The relative quantitative levels of IRF1 mRNA were 2.17fold higher in pSS patients than controls.IRF1 positive cells of the pSS group were localized in the epithelial islands,lymphocytes and ductal epithelial cells of the parotid glands.In all controls,the IRF1 positive cells were only localized on the ductal epithelial cells of parotid glands as determined by immunohistochemical staining or immunofluorescence.CONCLUSIONS： These findings indicate that IRF1 mRNA levels are up-regulated in the peripheral blood cells of pSS patients.Also,IRF1 positive cells exist in the epithelial islands,lymphocytes and ductal epithelial cells of the parotid glands of pSS,but are limited to the ductal epithelial cells of healthy controls.