Effect of a single application of TiF4 and NaF varnishes and solutions on dentin erosion in vitro

Objectives

This in vitro study aimed to analyse the effect of a single application of TiF₄ and NaF varnishes and solutions to protect against dentin erosion.

Methods

Bovine root dentin samples were pre-treated with NaF-Duraphat varnish (2.26%F, pH 4.5), NaF/CaF₂-Duofluorid varnish (5.63%F, pH 8.0), NaF-experimental varnish (2.45%F, pH 4.5), TiF₄-experimental varnish (2.45%F, pH 1.2), NaF solution (2.26%F, pH 4.5), TiF₄ solution (2.45%F, pH 1.2) and placebo varnish (pH 5.0, no-F varnish control). Controls remained untreated. Ten samples in each group were then subjected to an erosive demineralisation (Sprite Zero, 4× 90 s/day) and remineralisation (artificial saliva, between the erosive cycles) cycling for 5 days. Dentin loss was measured profilometrically after pre-treatment and after 1, 3 and 5 days of de-remineralisation cycling. The data were statistically analysed by two-way ANOVA and Bonferroni's post hoc test (p < 0.05).

Results

After pre-treatment, TiF₄ solution significantly induced surface loss (1.08 ± 0.53 μm). Only Duraphat reduced the dentin loss overtime, but it did not significantly differ from placebo varnish (at 3rd and 5th days) and TiF₄ varnish (at 3rd day).

Conclusions

Duraphat varnish seems to be the best option to partially reduce dentin erosion. However, the maintenance of the effects of this treatment after successive erosive challenges is limited.