Exposure of human skin to benzo[a]pyrene: Role of CYP1A1 and aryl hydrocarbon receptor in oxidative stress generation |
| |
| Authors: | C. Costa S. Catania R. De Pasquale R. Stancanelli G.M. Scribano A. Melchini |
| |
| Affiliation: | 1. Dip. Medicina Sociale del Territorio, Sez. Medicina del Lavoro-Università di Messina, A.O.U. “Policlinico G. Martino”, pad. H, via Consolare Valeria, 1 - 98125 Messina, Italy;2. Centro Interdip. di Tossicologia Sperimentale, Ambientale e del Lavoro (CITSAL) - Università di Messina, A.O.U. “Policlinico G. Martino”, pad. H, via Consolare Valeria, 1 - 98125 Messina, Italy;3. Dip. Farmaco-Biologico, Facoltà di Farmacia-Università di Messina, Vill. SS. Annunziata, 98168 Messina, Italy;4. Dip. Farmaco-Chimico, Facoltà di Farmacia-Università di Messina, Vill. SS. Annunziata, 98168 Messina, Italy |
| |
| Abstract: | Exposure to benzo[a]pyrene (BaP) can induce inflammatory skin diseases and skin cancer, which are both associated to oxidative stress. BaP is known to bind with high specificity to the aryl hydrocarbon receptor (AhR), modifying the expression of CYP1A1, involved both in cancer and inflammation. |
| |
| Keywords: | BaP, benzo[a]pyrene PAHs, polycyclic aromatic hydrocarbons AhR, aryl hydrocarbon receptor CYP1A1, cytochrome P450 1A1 COX-2, cyclooxygenase-2 ROS, reactive oxygen species OECD, organization for economic co-operation and development PBS, phosphate buffered saline MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide DCFDA, 2,7-dichlorofluorescein diacetate PIPES, piperazine-1,4-bis(2-ethanesulfonic acid) EDTA, ethylendiaminotetraacetic acid EGTA, ethylene glycol tetraacetic acid DTT, dithiothreitol PMSF, phenylmethylsulphonyl fluoride |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
