|
|||||
|
|
| 神经再生条件液中活性蛋白的分离和检测 | |
| 引用本文： | 李青峰,徐鲁平,景乃禾,姜文跃,平萍. 神经再生条件液中活性蛋白的分离和检测[J]. 中国修复重建外科杂志, 1999, 13(4): 199-201 |
| 作者姓名： | 李青峰 徐鲁平 景乃禾 姜文跃 平萍 |
| 作者单位： | 1. 上海第二医科大学附属第九人民医院整复外科,上海,200011 2. 中国科学院上海生物化学研究所 3. 中国科学院上海脑研究所 |
| 摘 要： | 目的了解神经再生条件液的生物活性。方法以ＳＤ大鼠的坐骨神经损伤后建立神经再生室模型，采用自然胶电泳分离法，将分离的各蛋白带分别与新生ＳＤ大鼠的后根神经节进行共同培养。结果应用自然胶电泳分离，神经再生条件液在分子量为６７～６６９ｋｕ区域有８条蛋白带。其中分子量在２３２～４４０ｋｕ区域的蛋白条带具有神经营养和趋化作用。结论神经再生条件液有促进神经再生的作用。 |
| 关 键 词： | 神经再生 神经再生条件液 自然胶电泳 |
THE SEPERATION AND DETECTION OF THE BIOACTIVE PROTEINS IN NERVE REGERATION CONDITIONED FLUIDS |
|
| /LI Qing feng,XU Lu ping,JING Nai he,et al.. THE SEPERATION AND DETECTION OF THE BIOACTIVE PROTEINS IN NERVE REGERATION CONDITIONED FLUIDS[J]. Chinese journal of reparative and reconstructive surgery, 1999, 13(4): 199-201 | |
| Authors: | /LI Qing feng XU Lu ping JING Nai he et al. |
| Affiliation: | Department of Plastic Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011. |
| Abstract: | OBJECTIVE: To understand the biological activities of the nerve regeneration conditioned fluid (NRCF). METHODS: Nerve regeneration chamber was made by using silicone tube bridging distal and proximal ends of severed SD rat's sciatic nerve. The biological activities of the proteins in NRCF, which were separated by natural polyacrylamide gel electrophoresis (PAGE), were analysed by being cocultured with excised neonatal dorsal root ganglia (DRG). RESULTS: Eight separated protein bands of NRCF were observed between 67-669 ku in molecular weight, and the protein bands between 232-440 ku showed strong neurotrophic and chemotactic function. CONCLUSION: NRCF has the promoting effects on nerve regeneration. |
| Keywords: | Nerve regeneration Nerve regeneration conditioned fluids Natural PAGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |