| 反相制备色谱分离酪丝亮肽拟似物 |
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| 引用本文： | 刘艺,孙迎基,张奕华,沈子龙. 反相制备色谱分离酪丝亮肽拟似物[J]. 药物生物技术, 2006, 13(4): 271-273 |
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| 作者姓名： | 刘艺 孙迎基 张奕华 沈子龙 |
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| 作者单位： | 1. 新药研究中心,中国药科大学,南京,210009
2. 生物技术中心,中国药科大学,南京,210009 |
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| 摘 要： | 本文介绍了以反相C18制备色谱柱分离强极性小肽拟似物的方法。采用BuCHI中压制备系统，以水-甲醇为梯度洗脱体系，并配合HPLC检测，结果成功分离纯化了三个活性拟肽化合物，纯度达97％以上。该法简便、分离效果好，可用于该类小肽化合物的分离制备。
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| 关 键 词： | 反相制备色谱 肽拟似物 |
| 文章编号： | 1005-8915（2006）04-0271-03 |
| 收稿时间： | 2005-10-07 |
| 修稿时间： | 2005-10-072006-04-10 |
Purification of Tyroserleutide Peptidomimetics by Reversed Phase Preparative Chromatography |
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| LIU Yi,SUN Ying-ji,ZHANG Yi-hua,SHEN Zi-long. Purification of Tyroserleutide Peptidomimetics by Reversed Phase Preparative Chromatography[J]. Pharmaceutical Biotechnology, 2006, 13(4): 271-273 |
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| Authors: | LIU Yi SUN Ying-ji ZHANG Yi-hua SHEN Zi-long |
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| Affiliation: | 1. Center of Drug Discovery ; 2. Biotechnical Research Center, China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | A method is described for the separation of strong-polar short peptidomimetics by reversedphase C18 preparative chromatographic column. Bu CHI middle pressure preparative system was applied to automatically control the experiments, using water-methanol as the gradient elution system. The purity of the eluate was validated by HPLC, and three short peptidomimetics were purified which reached 97% purity. The method is simple and effective. It can be applied to separate short peptidomimetics. |
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| Keywords: | Reversed-phase preparative chromatography Peptidomimetics |
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