依托咪酯对糖尿病大鼠肾脏缺血再灌注后心肌的影响

目的观察糖尿病大鼠肾缺血再灌注对远隔器官心肌组织的损伤作用,探讨依托咪酯对心肌组织的保护作用。方法健康成年雄性wistar大鼠36只,体质量300～350g,用链脲佐菌素(STZ)诱发大鼠糖尿病。糖尿病大鼠随机分为A组假手术组(n=12),B组肾缺血再灌注组(n=12),C组肾缺血再灌注+依托咪酯组(n=12),实验结束,24h后处死大鼠,光镜观察心肌组织的形态学改变,采用免疫组织化学法对TNF-α和NF-κB进行定性、半定量检测。结果与A组相比,B组大鼠心肌组织TNF-α和NF-κB表达上调(P<0.05),C组大鼠心肌组织TNF-α和NF-κB表达差异无统计学意义(P>0.05),与B组相比较,C组大鼠心肌组织TNF-α和NF-κB表达下调(P<0.05)。结论糖尿病大鼠肾缺血再灌注继发心肌组织损伤,依托咪酯能够明显减轻这种损伤,其保护作用可能与TNF-α表达抑制,TNF-α/NF-κB正反馈信号阻断有关。

Effect of Etomidate on Myocardium Injury Following Ischemia Reperfusion of Renal in Rat Model with Diabetic Nephropathy

Objective To investigate the protective effects of Etomidate against myocardiurn injury following renal iscbemia-reperfusion in rats with diabetic nephropathy. Methods Thirty-six healthy male Wistar rats weighing 300~350g, were induced to diabetic rats with streptozotocin. The diabetic rats were randomly assigned into three groups ： sham operation group （group A, n=12）, renal ischemia-reperfusion （group B, n=12）, renal ischemiareperfusion＋etomidate （group C, n=12）, end of the experiment, rats were sacrificed after 24 hours, the expression of TNF-α and NF-κB in myocardium were detected immunohistochemical and semi-quaititated by image analysis. Results The expression of TNF-α and NF-κB in group B, were significantly higher than in group A （P〈0.05） , and group C no significant difference to group A; the expression of TNF-α and NF-κB in group C were significantly lower than in group B. Conclusion Etomidate can attenuate the myocardium injury was induced by renal ischemia reperfusion indiabetic rats. It may be connecting with lower expression of TNF-α after the renal ischemia reperfusion and TNF-α/NF-κB positive feedback signal blocker.