高效液相色谱-电喷雾四级杆飞行时间质谱分析生姜中的姜辣素类化合物

建立高效液相色谱—电喷雾四级杆飞行时间质谱快速分析鉴定生姜中姜辣素类化合物的方法.采用Inertsil ODS-SP色谱柱(4.6 mm × 250 mm,5μm)分析,二元线性梯度洗脱,柱后流出液采用电喷雾四级杆飞行时间质谱正离子模式检测.根据电喷雾四级杆飞行时间质谱给出的母离子及二级碎片离子精确相对分子质量信息,结合相关文献,从生姜中分析鉴定了25种姜辣素,并用对照品对方法进行了验证.本方法快速、准确,可用于生姜中姜辣素类化合物的快速分析.

Analysis of gingerol-related compounds in fresh ginger by HPLC-ESI-Q-TOF-MS/MS

To establish a rapid method for analysis of gingerol-related compounds in fresh ginger by using high performance liquid chromatography coupled with electron spray ionization-quadrupole-time of flight mass/mass spectrometry (HPLC-ESI-Q-TOF-MS/ MS). The gingerol-related compounds in fresh ginger was separated by an Inertsil ODS-SP column (4.6 mm x 250 mm, 5 microm) using a binary eluent under gradient conditions. The analytes were detected by ESI-Q-TOF-MS/MS in positive ion mode to obtain MS and MS/MS spectra and to extract molecular weights. From the MS data, the accurate molecular weights of gingerol-related compounds were obtained, and from the MS/MS data, the (+) ESI-Q-TOF-MS/MS fragments were obtained. 25 gingerol-related compounds were identified from fresh ginger by attentive studying on the mass spectra of compounds and comparing with reference data reported in the literature, respectively. This method was certified to be accurate and reliable and can be used for the rapid analysis of gingerol-related compounds in fresh ginger.