| 人脐静脉内皮细胞的原代培养 |
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| 引用本文： | 王晓霞,牛勃,程牛亮,解军,张栋. 人脐静脉内皮细胞的原代培养[J]. 山西医科大学学报, 2007, 38(11): 1052-1053 |
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| 作者姓名： | 王晓霞 牛勃 程牛亮 解军 张栋 |
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| 作者单位： | 1. 山西医科大学生物化学与分子生物学教研室,太原,030001
2. 山西医科大学生物化学与分子生物学教研室,太原,030001;首都儿科研究所 |
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| 摘 要： | 目的建立一种能大量分离人脐静脉内皮细胞(HUVEC)且操作方便的体外培养方法。方法采用不同浓度的Ⅱ型胶原酶(0.05%,0.1%和0.2%)对HUVEC进行灌注,并分别孵育不同的时间(10min,13min,15min和18min)进行消化,观察细胞的形态、数量以及生长融合情况。结果采用0.1%的胶原酶消化10-13min为最佳条件,经光学显微镜观察培养的细胞数量较多,生长、繁殖状态良好,培养3d左右细胞可达到融合状态。结论本实验建立的胶原酶法消化HUVEC培养方法简便,细胞获得量较多且生长状态良好。
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| 关 键 词： | 细胞培养技术 人脐静脉 内皮细胞 |
| 文章编号： | 1007-6611（2007）11-1052-02 |
| 修稿时间： | 2007-08-31 |
Primary culture of human umbilical vein endothelial cells |
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| WANG Xiao-xia, NIU Bo, CHENG Niu-liang, et al. Primary culture of human umbilical vein endothelial cells[J]. Journal of Shanxi Medical University, 2007, 38(11): 1052-1053 |
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| Authors: | WANG Xiao-xia NIU Bo CHENG Niu-liang et al |
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| Affiliation: | Dept of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001 , China |
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| Abstract: | Objective To establish a technique for culturing human umbilical vein endothelial cells(HUVEC) in vitro.Methods Fresh HUVEC from human umbilical cords were perfused by different concentrations of collagenase and digested for different times.The morphology,numbers and fusion of cultured cells were examined by light microscopy.Results Digestion with 0.1% collagenase and incubation for 10-13 min were the best conditions for culturing cells.The cells grew to form confluent monolayers of polygonal cells within 3 d.Conclusion The digestion using collagenase is simple and successful for collection of HUVEC. |
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| Keywords: | cell culture techniques human umbilical vein endothelial cells |
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