氧化锆陶瓷表面粗糙度对微生物附着的影响

目的 分析牙科氧化锆陶瓷表面粗糙度对微生物附着的影响. 方法 使用碳化硅砂纸将氧化锆陶瓷表面逐级打磨为粗糙、中等粗糙和平滑表面,使用原子力显微镜和光学接触角测量仪测定表面粗糙度、形态及接触角,通过使用变异链球菌UA159处理的探针检测其与不同粗糙度氧化锆陶瓷表面的粘附力;并将氧化锆陶瓷与变异链球菌共培养,通过扫描电镜和激光共聚焦显微镜观察生物膜形成情况. 结果 不同粗糙度表面的氧化锆陶瓷,由粗糙到平滑,接触角逐渐减小,表面能逐渐增大,表面生物膜厚度逐渐减小,变异链球菌与氧化锆陶瓷表面的粘附力减小,但生物膜细菌量差异无统计学意义(P>0. 05). 结论 随着氧化锆表面粗糙度增加,变异链球菌的粘附力增加;随着粗糙度减小,生物膜厚度与微生物数量相应减小,但表面粗糙度减小至一定数值后,生物膜厚度与微生物数量无显著变化.

The influence of the roughness of zirconia ceramic surface on microbial attachment

Objective To analyze the influence of the zirconia ceramic surface roughness on microbial attachment. Methods The surface of zirconia ceramics was abraded progressively into three roughness levels of rough, medium rough and smooth surface by silicon carbide sand papers. Then the surface roughness, surface morphology and contact angle of zirconia ceramic were measured by atomic force microscopy and optical contact angle measuring device, which was fol-lowed by detecting the adhesive force of different roughness of zirconia ceramic by Streptococcus mutans UA159 prepro-cessed probe. At last scanning electron microscope and confocal laser scanning microscopy were used to observe biofilm formation after co-culture of zirconia ceramics and Streptococcus mutans. Results For different roughness of zirconia ce-ramic surface, with the smoother degree of their surfaces, the contact angle decreases step by step, surface energy increa-ses gradually, surface biofilm thickness decreases generally, and adhesive force between Streptococcus mutans and surface of zirconia ceramics decreases by degrees, whereas the biofilm bacteria mass of different roughness of zirconia ceramics does not have statistical difference. Conclusion Zirconia surface roughness affects the adhesion force of Streptococcus mutans which grows with the increase of surface roughness. The biofilm thickness and the mass of microorganisms less-ened correspondingly with the decreasing of surface roughness, until it drops to a certain number, from where the biofilm thickness and the mass of microorganisms remains stable.