Calbindin-D 28K in hippocampal organotypic cultures |
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| Authors: | N Bousez-Dumesnil M Thomasset Y Ben-Ari |
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| Affiliation: | I.N.S.E.R.M. U. 29, H?pital de Port-Royal, Paris, France. |
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| Abstract: | Slices of hippocampus from 6-day-old rats were cultured for 2-4 weeks using the roller-tube technique. The organization of these explants was studied by immunocytochemical labeling of calbindin-D 28K (CaBP 28K). The development of the CaBP 28K staining was very close to that of the rat hippocampus in vivo with only 3 subpopulations of labeled cells: granule cells and their mossy fibers, pyramidal cells in the subiculum-CA1 zone and interneurons scattered in strata oriens and radiatum. |
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