经皮微波凝固治疗肝癌对患者局部免疫状态的影响

目的 探讨超声引导下经皮微波凝固治疗肝癌对患者局部免疫状态的影响。方法 ７８例经病理检查证实为肝癌并自愿接受ＰＭＣＴ者于治疗前,治疗后３ｄ、１７ｄ和３０ｄ在超声引导下经皮作肝脏穿刺,取癌灶内及其周围肝组织做免疫组化染色,观察并计数癌灶内、外ＣＤ３、ＣＤ４５ＲＯ、ＣＤ５６、ＣＤ６８和ＣＤ２０阳性细胞数。结果 治疗前癌灶内、外有不同程度的上述细胞浸润。这些细胞主要散布于癌周的肝窦及癌基质内,治疗后３ｄ

Effect of percutaneous microwave coagulation therapy on local immune response in patients with hepatocellular carcinoma

Objective To assess the local immune response to percutaneous microwave coagulation therapy (PMCT) for hepatocellular carcinoma (HCC). Methods Seventy eight patients with HCC underwent PMCT. Samples of cancer and adjacent liver tissues were taken at pre therapy, and 3, 17 and 30 days after therapy by ultrasound guided biopsy and were stained by immunohistochemical technique. The CD3, CD45RO, CD56, CD68 and CD20 positive cells were detected in the tissue section and counted under a microscope. Results There were some CD3, CD45RO, CD56, CD68 and CD20 positive cells scattered among the cancer stroma and sinusoids and surrounding liver tissue pre therapy. Those cells increased significantly at the third day post therapy compared with those of pre therapy both in cancer and adjacent liver tissues, except CD20 positive cells. The number of infiltrating immunocytes was related to the basic immune state of patients ( r =0.56, P =0.013). Those immunocytes scattered among the parenchymal area of the tumor, the lumen of small blood vessels and the stroma closing to small vessels. The number of CD45RO and CD56 cells reached their highest point at the day 17 and CD68 positive cells at day 3 and sustained to the day 30 after PMCT. Those immunocytes were seen among the cancer cells and the lumen of small blood vessels after. Conclusion The number of immunocytes in the lesion area is increased and the local immune function is enchanced by PMCT.