人参皂苷Rh2对低浓度的奥沙利铂诱导人胃癌SGC-7901侧群细胞凋亡的增敏作用

目的:探讨人参皂苷Rh2（GS-Rh2）是否可以提高胃癌细胞对奥沙利铂（L-OHP）药物作用的敏感性。方法:使用流式细胞仪检测并分选胃癌SGC-7901的侧群（SP）细胞，使用CCK-8试剂盒检测并验证SP细胞的高增殖能力，使用MTT法检测GS-Rh2和L-OHP对SGC-7901-SP细胞的半数抑制率（IC₅₀），通过流式细胞仪检测GS-Rh2联合低浓度的L-OHP干预后SGC-7901-SP细胞周期及凋亡率的变化，使用Western blotting检测GS-Rh2和L-OHP干预后SGC-7901-SP细胞的凋亡蛋白Bax、Bcl-2表达变化情况。结果:胃癌SGC-7901-SP细胞的增殖能力明显高于非侧群细胞（P<0.01）；GS-Rh2能增强L-OHP对SGC-7901-SP细胞的敏感性，将细胞增殖阻滞在G₁期（P>0.05），其Bax蛋白表达增高（P<0.01），Bcl-2蛋白表达降低（P<0.01），并与GS-Rh2浓度呈负相关（P<0.05）。结论:随着GS-Rh2浓度的逐渐增加，GS-Rh2逐渐提高了L-OHP对SGC-7901-SP作用的的敏感性，GS-Rh2可作为一种抗肿瘤药物的增敏剂。

Sensitization effect of ginsenoside-Rh2 on the apoptosis of human gastric cancer SGC-7901 side population cells induced by low concentration of oxaliplatin

Objective: To explore the effects of ginsenoside-Rh2(GS-Rh2) on the sensitivity of gastric cancer cells treated with oxaliplatin(L-OHP).Methods: The side population(SP) cells of human gastric cancers cell line SGC-7901 was screened using flow cytometry(FCM),the proliferation ability of SP cells was detected using CCK-8 kit.The half inhibition concentration(IC₅₀) of GS-Rh2 and L-OHP on SGC-7901 SP cells were detected using MTT assay.The changes of cell cycle and apoptosis rate of SP cells intervened by GS-Rh2 combined with low concentration of L-OHP were detected using FCM,and the level changes of Bax and Bcl-2 protein in SP cells intervened by GS-Rh2 combined with L-OHP were measured using Western-blotting.Results: The proliferation capacity of SGC-7901 SP cells was significantly higher than that of non side population(P<0.01).GS-Rh2 could enhance the sensitivity of the L-OHP on SGC-7901-SP cells,block the cell cycle in G₁ phase(P>0.05).The protein expressions of Bax and Bcl-2 increased and decreased,respectively(P<0.01),which was negatively correlated with the GS-Rh2 concentration(P<0.05).Conclusions: With the increasing of GS-Rh2 concentration,the GS-Rh2 gradually increases the sensitivity of L-OHP on SGC-7901 SP cells.GS-Rh2 can be acted as a sensitizer of anti-gastric cancer drug.