RNA干扰FAK表达抑制宫颈癌细胞侵袭、迁移能力的研究

目的 探讨黏着斑激酶(FAK)表达量降低对宫颈癌细胞侵袭、迁移能力的影响以及机制研究.方法以宫颈癌HeLa细胞为研究对象,转染RNA干扰载体,实验分3组:空白对照组(未做任何处理的细胞)、阴性对照组(细胞转染对照载体FAK-pGenesil-Ctrl)、干扰组(细胞转染重组质粒FAK-pGenesil-siRNA).Transwell法检测细胞的迁移、侵袭能力,Western blot法检测细胞中的FAK、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9 (MMP-9)、上皮细胞钙黏蛋白(E-cadherin)的蛋白表达量.结果 干扰组细胞中FAK蛋白的表达量低于空白对照组和阴性对照组(P﹤0.05);干扰组细胞的迁移、侵袭数少于空白对照组和阴性对照组(P﹤0.05);干扰组细胞中MMP-2、MMP-9蛋白的表达量低于空白对照组和阴性对照组,但E-cadherin蛋白的表达量高于空白对照组和阴性对照组(P﹤0.05).结论 RNA干扰FAK的表达量可以降低宫颈癌HeLa细胞的侵袭、迁移能力,其作用机制可能是通过影响细胞上皮间质转化相关因子MMP-2、MMP-9、E-cadherin蛋白的表达量.

Reversal effect of RNA interference targeting FAK on the migration and invasion of cervical cancer cells

Objective To investigate the effect and mechanism of FAK on the invasion and migration of cervical can-cer cells.Method Cervical cancer cells HeLa were used in the study,the cells were transfected with RNAi interfering vector,and then were divided as 3 groups:blank control group(untreated cells);negative control group(transfected with FAK-pGenesil-Ctrl vector);interference group(transfected with recombinant plasmid FAK-pGenesil-siRNA).Transwell was applied to determine the migration and invasion of cells,while the expression of FAK,MMP-2,MMP-9 and E-cad-herin protein in the cells were detected by Western blot.Result The expression of FAK protein in the interference group was significantly lower than that in the blank control group and the negative control group(P<0.05);lower ability of mi-gration and invasion of the cells was observed in interference group than in the blank control group and the negative con-trol group(P<0.05);the expression of MMP-2 and MMP-9 in interference group were significantly lower than that of the control group and the negative control group,but the expression of E-cadherin was significantly higher than that of blank control group and negative control group(P<0.05).Conclusion The expression of FAK interfered by RNA may lead to decreased ability of invasion,and migration of cervical cancer cells HeLa,which be related to the expression epithelial-mesenchymal transformation-related factors,including MMP-2,MMP-9 and E-cadherin protein.