miRNA-145、Ki-67、survivin、Aurora-b在喉鳞状细胞癌中的表达及临床意义

目的 探讨miRNA-145、Ki-67、生存素(survivin)、Aurora-b在喉鳞状细胞癌(LSCC)中的表达及临床意义.方法 采用实时荧光定量PCR(RT-PCR)检测85例LSCC组织及85例癌旁正常组织中miRNA-145的表达量,采用免疫组化法检测LSCC组织中Ki-67、survivin、Aurora-b的表达水平,分析miRNA-145、Ki-67、survivin、Au-rora-b表达情况与LSCC患者临床特征的关系及四者的相关性.结果 LSCC组织中miRNA-145的表达量明显低于癌旁正常组织(P﹤0.01).LSCC组织中Ki-67、survivin、Aurora-b的阳性表达率明显高于癌旁正常组织,miRNA-145的阳性表达率明显低于癌旁正常组织,差异均有统计学意义(P﹤0.01).miRNA-145、Ki-67、survivin、Aurora-b的阳性表达率与LSCC患者的TNM分期和淋巴结转移情况有关(P﹤0.01),而与患者的年龄、性别、肿瘤直径及分化程度无关(P﹥0.05).LSCC组织中miRNA-145与Ki-67、survivin、Aurora-b的表达均呈负相关(P﹤0.01),Ki-67、survivin及Aurora-b的表达两两之间呈正相关(P﹤0.01).结论 miRNA-145在LSCC组织中低表达,Ki-67、survivin、Aurora-b在LSCC组织中高表达,miRNA-145、Ki-67、survivin、Aurora-b的阳性表达率与LSCC患者的TNM分期和淋巴结转移情况密切相关,四者联合检测可能成为LSCC诊断的有效指标.

Clinical significance and expression of miRNA-145, Ki-67, survivin and Aurora-b in laryngeal squamous cell carcinoma

Objective To explore the clinical significance and expression of miRNA-145, Ki-67, survivin and Aurora-b in laryngeal squamous cell carcinoma (LSCC). Method The expressions of miRNA-145 in 85 cases of LSCC and cor-responding adjacent normal tissues were detected by Real-Time PCR. The expressions of Ki-67, survivin and Aurora-b in LSCC and adjacent normal tissues were detected by immunohistochemical method. The relationships among miRNA-145, Ki-67, survivin, Aurora-b and clinical features, as well as the correlation of the four, were analyzed. Result The expres-sion of miRNA-145 in LSCC was significantly lower than that in adjacent normal tissues (P<0.01), the positive expres-sion rates of Ki-67, survivin and Aurora-b in LSCC were significantly higher than those in adjacent normal tissues (P<0.01), respectively;the positive rate of miRNA-145 was significantly lower than that in adjacent normal tissues (P<0.01). The expressions of miRNA-145, Ki-67, survivin and Aurora-b in LSCC were associated with TNM stage and lymph node metastasis (P<0.01), but not related to age, sex, tumor diameter and differentiation degree (P>0.05). At the same time, the expression of miRNA-145 in LSCC was negatively correlated with the expressions of Ki-67, survivin and Aurora-b (P<0.01). Positive correlation was found between the expression of Ki-67, survivin and Aurora-b in LSCC in pairwise com-parison (P<0.01). Conclusion The low expression of miRNA-145, high expressions of Ki-67, survivin and Aurora-b in LSCC tissue are associated with the TNM stage and lymph node metastasis of LSCC, and the combined result of expres-sions of the four might become an effective indicator for the diagnosis of LSCC.