纤维支气管镜检标本微小RNA-21在非小细胞肺癌诊断中的临床应用价值

目的 探讨纤维支气管镜检标本微小RNA-21(miRNA-21)在非小细胞肺癌(NSCLC)诊断中的临床应用价值.方法 选取80例NSCLC患者作为NSCLC组,选取同期接受镜检筛查的80例肺炎或肺结核患者作为对照组.检测两组患者的镜检物miRNA-21表达水平,比较两组患者的细胞因子和肿瘤标志物水平,分析NSCLC患者镜检物miRNA-21水平与细胞因子和肿瘤标志物水平的相关性.结果 NSCLC组患者的镜检物miRNA-21水平为(8.29±1.15),明显高于对照组的(1.35±0.41),差异有统计学意义(P﹤0.001).NSCLC组患者的γ-干扰素(IFN-γ)、白细胞介素-1β(IL-1β)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平明显高于对照组,差异均有统计学意义(P﹤0.001).NSCLC组患者的癌胚抗原(CEA)、神经元特异烯醇化酶(NSE)和角蛋白CYFRA21-1水平明显高于对照组,差异均有统计学意义(P﹤0.001).NSCLC患者的镜检物miRNA-21表达水平与细胞因子IFN-γ、IL-1β、IL-4、IL-6、TNF-α和肿瘤标志物CEA、NSE、CYFRA21-1水平均呈正相关(P﹤0.05).结论 NSCLC患者纤维支气管镜检标本中miRNA-21的表达水平较高,且与细胞因子和肿瘤标志物水平密切相关.

Clinical value of microRNA-21 level in the diagnosis of non-small cell lung cancer by fiber-optic bronchoscopy

Objective To observe the expression of microRNA-21(miRNA-21)in patients with non-small cell lung cancer (NSCLC) and to analyze its clinical significance. Method 80 patients with NSCLC were included as NSCLC group,meanwhile,another 80 cases with pneumonitis or tuberculosis who underwent fiber-optic bronchoscopy were re-cruited as control group.The level of miRNA-21 in the two groups was observed microscopically,the level of cytokines and tumor markers of the two groups were compared,and then the correlation between miRNA-21 level and cytokines and tumor markers in NSCLC patients was analyzed.Result The level of miRNA-21 in the NSCLC group was(8.29± 1.15),which was significantly higher than that of the control group at(1.35±0.41)(P<0.001).The levels of IFN-γ,IL-1β, IL-4,IL-6 and TNF-α in NSCLC group were higher than those in the control group,with significant difference observed (P<0.001).The levels of CEA,NSE and CYFRA21-1 in NSCLC group were higher than those in the control group,dem-onstrating statistically significant difference(P<0.001).The level of miRNA-21 in the patients with NSCLC was positive-ly correlated with the level of cytokines which were IFN-γ、IL-1β、IL-4、IL-6、TNF-α and tumor markers of CEA,NSE, CYFRA21-1(P<0.05).Conclusion The miRNA-21 in the specimen of NSCLC patients with fiber-optic bronchoscopy is highly expressed,and is closely related to the level of cytokines and tumor markers.