吡格列酮对2型糖尿病大鼠胰岛素抵抗和胰岛功能的影响

目的:建立2型糖尿病（T2DM）的大鼠模型，观察吡格列酮（PIO）对胰岛素抵抗和胰岛功能的影响。方法:SD雄性大鼠40只，随机分为对照组（10只）和高脂组（30只）。高脂组建立2型糖尿病大鼠模型，得到T2DM大鼠24只。将成模的24只T2DM大鼠随机分为糖尿病组（DM组）和PIO组，每组12只。监测DM组、PIO组和对照组大鼠体质量、空腹血糖、空腹胰岛素、糖化血红蛋白、超敏C反应蛋白以及血脂，计算胰岛素抵抗指数、胰岛β细胞功能指数（HOMA-β）以及胰岛素敏感指数。结果:DM组、PIO组大鼠的体质量、总胆固醇、三酰甘油、低密度脂蛋白胆固醇、空腹血糖、空腹胰岛素、糖化血红蛋白、超敏C反应蛋白、胰岛素抵抗指数均高于对照组（P<0.05~P<0.01），高密度脂蛋白胆固醇、胰岛素敏感指数和HOMA-β则均低于对照组（P<0.05~P<0.01）；PIO组的HOMA-β高于DM组（P<0.05）。结论:PIO发挥治疗T2DM大鼠糖尿病的作用可能与减轻T2DM大鼠的胰岛素抵抗和改善胰岛功能有关。

Effect of pioglitazone on the insulin resistance and function of pancreatic islet in Type 2 diabetes rat

Objective:To establish the type 2 diabetes mellitus(T2DM) rat model,and observe the effects of pioglitazone on insulin resistance and function of pancreatic islets in T2DM rats.Methods:Forty SD male rats were randomly divided into the control group (NC group,10 rats) and high-fat group (30 rats).Twenty-four T2DM model rats were established,and randomly divided into the diabetes mellitus group (DM group) and PIO group (12 rats in each group).The levels of body mass (BM),fasting blood glucose (FBG),fasting insulin (FINS),glycosylated hemoglobin hemoglobin-A1 c (HbA1c),C-reactive protein (CRP),blood lipid,insulin resistance index,islet β-cell function index and insulin sensitivity index(ISI) were measured in two groups.Results:The levels of BM,TC,TG,LDL-C,FBG,HbA 1 c,FINS/CRP and HOMA-β index in DM group and PIO group were higher than those in control group(P ＜0.05 to P ＜0.01).The levels of HDL-C,ISI and HOMA-β in DM group and PIO group were lower than those in control group(P ＜0.05 to P ＜ 0.01),and the level of HOMA-β in PIO group was higher than that in DM group (P ＜ 0.05).Conclusions:Pioglitazone plays a therapeutic role in T2DM rats,which may be involved in reducing insulin resistance and improving islet function.