外周血T细胞亚群计数与恶性肿瘤的相关性研究

目的:探讨外周血T细胞亚群计数在恶性肿瘤中临床意义。方法:采用流式细胞术检测80例恶性肿瘤病人和100名健康体检者外周血CD3+、CD4+和CD8+细胞计数以及CD4+/CD8+比值。结果:恶性肿瘤病人CD3+、CD4+、CD8+细胞计数及CD4+/CD8+比值均较健康人群显著降低（P<0.01）。亚组分析结果显示，各恶性肿瘤组的CD3+和CD4+细胞计数均较对照组下降（P<0.05~P<0.01），CD8+细胞计数与对照组比较差异均无统计学意义（P>0.05），仅肺癌组CD4+/CD8+比值低于对照组（P<0.05）；首诊组和进展组恶性肿瘤病人的所有观察指标差异均无统计学意义（P>0.05）；男性恶性肿瘤病人的CD4+细胞计数低于女性病人（P<0.05）。结论:恶性肿瘤病人的CD3+、CD4+、CD8+细胞水平以及CD4+/CD8+比值存在一定程度的失衡，通过检测这些指标，可以了解病人机体的免疫状态，直接或者间接为肿瘤的免疫治疗提供依据。

Study on the correlation between T-lymphocyte subgroup count of peripheral blood and malignant tumor

Objective: To investigate the clinical significance of T-lymphocyte subgroup count of peripheral blood in malignant tumor.Methods: The amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ of peripheral blood in 80 malignant tumor patients and 100 healthy people were determined by flow cytometry.Results: Compared with healthy people,the amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in patients with malignant tumor were significantly decreased(P<0.01).The results of subgroup analysis showed that the counts of CD3+ cells and CD4+ cells in malignant tumor patients decreased compared with control group(P<0.05 to P<0.01),the difference of the count of CD8+ cells between two groups was not statistically significant(P>0.05),and ratio of CD4+/CD8+ in lung cancer group was lower than that in control group(P<0.05).The difference of the parameter of malignant tumor patients between the first diagnosis group and progression group was not statistically significant(P>0.05).The amount of CD4+ cells in amale patients was lower than that in female patients(P<0.05).Conclusions: The imbalance of the counts of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in malignant tumor patients are identified,which can be used to evaluate the immunity status of tumor patients,and provide a scientific evidence in immunotherapy of tumor.