按蚊对5种常用杀虫剂的抗药性现场调查研究

目的调查大连市媒介按蚊成蚊对DDT、溴氰菊酯、残杀威、高效氯氰菊酯、氯菊酯抗药性，为合理使用杀虫剂提供科学依据。方法采用WHO成蚊滤纸接触筒法，观察区分剂量开始击倒时间、不同时间击倒率和24h死亡率，根据死亡率判定抗性级别。结果大连市媒介按蚊成蚊对DDT、溴氰菊酯、残杀威、高效氯氰菊酯、氯菊酯首只蚊虫击倒时间分别为6,61、4、8、8min。区分剂量24h死亡率：依次为溴氰菊酯91．30％、残杀威89．48％、氯菊酯84．93％、高效氯氰菊酯80．43％为初步抗性群体（M）；DDT50．60％为抗性群体（R）。结论应加强媒介按蚊抗药性监测，并根据具体情况，采用适宜防蚊方法，合理地使用杀虫剂，预防或减缓媒介按蚊对杀虫剂抗药性的产生和发展。

Resistance of Anopheles to 5 kinds of common used insecticides in Dalian city

Objective To investigate the resistance of adult mosquito of Anopheles to DDT, deltamethrin, propoxur, beta cypermethrin and permethrin in Dalian and provide scientific evidence for its control. Methods Anopheles were captured with filter contact method recommended by WHO and the knockdown rate, the knockdown time and 24 h mortality were observed. Results The knockdown time of the first Anopheles to DDT,deltamethrin,propoxur, beta cypermethrin,permethrin in Dalian were 6,6,4,8,8 rain. Death rate of discrimination dosages ： dehamethrin 91.30%, propoxur 89.48%, permethrin 84.93 %, beta cypermethrin 80.43 %, they were initially resistant group （ M ） ; DDT 50.60 % was a resistant group （R）. Conclusion Resistance should be monitored regularly. Integrated use of insecticides and other methods for control of Anopheles should be carried out according to local conditions. The occurrence and development of resistance could be prevented and reduced by scientific and rational use of insecticides.