| PC12细胞缺糖损伤的分子生物学特征 |
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| 引用本文： | 宋晓冬,蒋少锋,牛新华,徐艳岩,武玉永,单长民,左伋. PC12细胞缺糖损伤的分子生物学特征[J]. 滨州医学院学报, 2006, 29(1): 9-11 |
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| 作者姓名： | 宋晓冬 蒋少锋 牛新华 徐艳岩 武玉永 单长民 左伋 |
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| 作者单位： | 滨州医学院生物学教研室,滨州市,256603;复旦大学医学院,上海市,230002 |
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| 摘 要： | 目的在体外模拟能量代谢障碍研究PC12细胞缺糖损伤的分子生物学特征.方法电镜观测形态改变,MTT检测功能异常,RT-PCR测定Bcl-2在RNA水平上的表达.结果电镜观察到细胞有凋亡现象;MTT显示细胞活力降低;RT-PCR结果表明Bcl-2在6～16 h表达上调.结论缺糖损伤的PC12细胞既有形态的变化也有功能上的变化,并同时伴随着凋亡和坏死两种死亡现象.
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| 关 键 词： | PC12细胞 缺糖损伤 凋亡 |
| 文章编号： | 1001-9510（2006）01-0009-03 |
| 收稿时间： | 2005-05-12 |
| 修稿时间： | 2005-05-12 |
Cellular and molecular alterations of PC12 cells deprived of glucose |
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| SONG Xiaodong ,JIANG Shaofeng, NIU Xinhua et al. Cellular and molecular alterations of PC12 cells deprived of glucose[J]. Journal of Binzhou Medical College, 2006, 29(1): 9-11 |
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| Authors: | SONG Xiaodong JIANG Shaofeng NIU Xinhua et al |
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| Affiliation: | Department of Biology, Binzhou Medical College, Binzhou 256603 |
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| Abstract: | Objective By using glucose deprivation (an insult relevant to the brain ischemia) media,PC12 cell strain was evaluated.Methods By cellular and molecular biological techniques including electron microscopy,MTT,and RT-PCR to meaure and observe.Results Electron microscopy reveals the treated PC12 cells underwent apoptosis;MTT result shows the treated PC12 cells viabilities induced;Bcl-2 RNA levels expressed to peak levels 6-16 hours after glucose deprivation treatment and then declined gradually.Conclusion The PC12 cells deprived of glucose underwent the morphological and functional alterations including apoptosis and necrosis. |
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| Keywords: | PC12 cell glucose deprivation apoptosis |
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