| siRNA靶向沉默MALAT-1基因对人食管癌EC9706细胞体外侵袭迁移的影响 |
| |
| 引用本文： | 张 璞,' target='_blank'>,薛 锋,周 斌.siRNA靶向沉默MALAT-1基因对人食管癌EC9706细胞体外侵袭迁移的影响[J].现代肿瘤医学,2015,0(10):1337-1340. |
| |
| 作者姓名： | 张 璞 ' target='_blank'> 薛 锋 周 斌 |
| |
| 作者单位： | 1.西安交通大学医学院，陕西 西安 710061;2.陕西核工业215医院，陕西 咸阳 712000;3.西安交通大学医学院第二附属医院，陕西 西安 710004 |
| |
| 基金项目： | 陕西省科学技术攻关项目（编号:2009k12-01） |
| |
| 摘 要： | 目的:研究长链非编码RNA MALAT-1对食管癌EC9706细胞系迁移、侵袭能力的影响。方法:化学合成针对MALAT-1的siRNA，脂质体法转染EC9706细胞，转染48h后RT-PCR和Western blot检测各细胞MALAT-1在mRNA和蛋白质水平的表达。Transwell实验检测迁移、侵袭能力改变。结果:小干扰RNA下调MALAT-1表达后，RT-PCR和Western blot证实食管癌EC9706细胞中MALAT-1的表达在mRNA水平和蛋白质水平均明显下调，Transwell实验证实食管癌EC9706细胞迁移、侵袭能力下降。结论:抑制MALAT-1表达能够使食管鳞癌细胞的侵袭转移能力明显降低。
|
| 关 键 词： | 食管鳞癌 MALAT-1 侵袭 迁移 |
The influence of silence MALAT-1 gene on the invasion and metastasis ability of human esophageal squamous cell carcinoma cell EC9706 |
| |
| Zhang Pu,' target='_blank'>,Xue Feng,Zhou Bin.The influence of silence MALAT-1 gene on the invasion and metastasis ability of human esophageal squamous cell carcinoma cell EC9706[J].Journal of Modern Oncology,2015,0(10):1337-1340. |
| |
| Authors: | Zhang Pu ' target='_blank'> Xue Feng Zhou Bin |
| |
| Institution: | 1.Xi'an Jiaotong University School of Medicine，Shaanxi Xi'an 710061,China;2.No.215 Hospital of Shaanxi Nudear Industry,Shaanxi Xianyang 712000,China;3.The Second Affiliated Hospital of Xi'an Jiaotong University School of Medicine,Shaanxi Xi'an 710004,China. |
| |
| Abstract: | Objective:To investigate the protective effects of MALAT-1 on regulation of migration and invasion in human esophageal squamous cell carcinoma (ESCC) cell line EC9706.Methods:The small interfering RNA (siRNA) was designed and synthesized and then transfected into the EC9706 cells.The transfection efficiency was detected with RT-PCR and Western blot assay.Transwell migration and matrigel invasion assays were performed to evaluate the migration and matrigel invasion respectively.Results:After down regulation of the expression of MALAT-1,the migration and matrigel invasion ability of EC9706 esophageal squamous cell carcinoma cells were impaired.Conclusion:MALAT-1 promoted the migration and matrigel invasion in EC9706 esophageal squamous cell carcinoma cells.Down regulate the expression of MALAT-1 can inhibit the invasion and metastasis ability of ESCC. |
| |
| Keywords: | esophageal squamous cell carcinoma MALAT-1 invasion metastasis |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
| 点击此处可从《现代肿瘤医学》下载免费的PDF全文 |
