胃腺癌患者外周血中CD4+T淋巴细胞表面抑制性分子PD-1的表达

目的:检测胃腺癌患者外周血中程序性死亡分子1（programmed death-1，PD-1）在CD4+T淋巴细胞上的表达水平，探讨其与胃腺癌的关系。方法:选取未经放、化疗或外科手术治疗的胃癌患者57例、疾病对照22例与健康对照20例，取新鲜抗凝血经流式细胞术（flowcytometry，FCM）检测抑制性分子PD-1的百分比例；同时对胃腺癌患者进行TNM分期比较。结果:胃腺癌患者外周血中PD-1在CD4+T淋巴细胞上的表达水平（10.91±4.36）%比疾病对照（8.14±3.65）%和健康对照（7.37±3.06）%显著升高，Ⅲ-Ⅳ期胃腺癌患者的CD4+PD-1+T细胞的百分比例（11.9±3.85）%显著高于Ⅰ-Ⅱ期（9.08±3.13）%。结论:抑制性分子PD-1在胃腺癌外周血中CD4+T淋巴细胞表面表达增加，并随胃腺癌进展而升高，提示CD4+PD-1+T淋巴细胞与胃腺癌的发生发展相关。

Expression of inhibitory molecule PD-1 on CD4+ T lymphocytes in peripheral blood of gastric adenocarcinoma patients increased

Objective:To detect the expression level of PD-1 on CD4+T lymphocytes in peripheral blood of gastric adenocarcinoma patients and investigate the relationship with progression of gastric adenocarcinoma.Methods:Anticoagulated fresh bloods of 57 gastric adenocarcinoma patients, 22 disease controls and 20 healthy controls were studied.The expression level of inhibitory molecule PD-1 was assayed by flowcytometry(FCM).Results:The percentage of CD4+PD-1+T cells were significantly higher in peripheral blood of gastric adenocarcinoma patients than in disease controls and healthy controls; The ratio of CD4+PD-1+T cells significantly increased in patients with stages Ⅲ and Ⅳgastric adenocarcinoma as compared with patients with stagesⅠand Ⅱgastric adenocarcinoma.Conclusion:The increased expression of inhibitory molecules PD-1 on CD4+T lymphocytes in peripheral blood of gastric adenocarcinoma patients, and increased with gastric adenocarcinoma progression, indicating that CD4+PD-1+T lymphocytes correlates with the occurrence and progression of gastric adenocarcinoma.