miR-542-5p对卵巢癌细胞SKOV3恶性生物学行为的影响

目的:探讨miR-542-5p对卵巢癌细胞系SKOV3的增殖、迁移、侵袭、凋亡等恶性生物学行为的影响。方法:应用miR-542-5p mimics和mimics阴性对照 (mimics NC)分别转染卵巢癌细胞SKOV3。采用MTT实验、划痕试验、Transwell实验和流式细胞术检测过表达miR-542-5p对SKOV3细胞增殖、迁移、侵袭、凋亡的影响。结果:过表达miR-542-5p后，SKOV3细胞的增殖能力较阴性对照和正常SKOV3细胞明显降低（P<0.05）；Transwell实验和划痕实验显示，过表达miR-542-5p后，细胞的迁移和侵袭能力较阴性对照组明显下降（P<0.05）；流式细胞术结果显示，过表达miR-542-5p后，SKOV3细胞的凋亡率较阴性对照组升高，而对细胞周期无影响。结论:过表达miR-542-5p可以抑制卵巢癌细胞SKOV3的体外增殖、迁移和侵袭能力，诱导细胞凋亡。提示miR-542-5p在卵巢癌恶性生物学进程中可能发挥重要作用。

The effect of miR - 542 - 5p on the malignant behavior of ovarian cancer cell line SKOV3

Objective:To investigate the effect of miR-542-5p on the cell proliferation，migration,invasion and apoptosis of ovarian cancer SKOV3 cell.Methods:miR-542-5p mimics and mimics negetive control (mimics NC) were transfected into SKOV3 cell lines.MTT assay，scratch test，Transwell assay and flow cytometry were performed to evaluate the effect of overexpression of miR-542-5p on cell proliferation，migration，invasion and cel1 apoptosis of SKOV3 cell line.Results:The ability of proliferation of SKOV3 cell was significantly decreased compared with the negetive control group and parental SKOV3 cell group after overexpression of miR-542-5p（P<0.05）.Transwell and scratch tests indicated that after overexpression of miR-542-5p，the invasion and migration ability of SKOV3 were significantly decreased than the negetive control group（P<0.05）.Flow cytometry showed that the apoptosis rate of SKOV3 cell was increased after overexpression of miR-542-5p compared with the negetive control group，but had no effect on cell cycle.Conclusion:The proliferation，migration and invasion of SKOV3 cell lines can be inhibited and the apoptosis rate can be increased by increasing the expression of miR-542-5p.Our results indicate that miR-542-5p may play an important role in the malignant phenotypes of ovarian cancer.