ADAM9基因干涉对非小细胞肺癌细胞（A549）体外及体内增殖的影响

目的:探讨解聚素金属蛋白酶9(ADAM9)基因对非小细胞肺癌细胞（A549）增殖的影响。方法:采用RT-PCR、免疫细胞化学、Western blot及ELISA法检测ADAM9基因在A549细胞中的表达；采用MTT法检测不同浓度ADAM9基因对A549细胞体外增殖及变化的影响； 观察不同浓度ADAM9基因干涉A549细胞在裸鼠体内成瘤后生长情况。结果:ADAM9基因在A549细胞中主要表达于细胞间质和细胞浆中，在细胞膜中的表达较少；人ADAM9基因对A549细胞干涉后，其增殖活性与对照组对比显著增强(P＜0.01)；人ADAM9基因对A549裸鼠体内成瘤后干涉显示，ADAM9基因对移植瘤增生促进较对照组显著（P＜0.05）。结论:人ADAM9基因对A549细胞体外干涉及其体内成瘤后干涉结果均有促进作用。

A549 cells proliferation are interfered by ADAM9 gene in vitro and in vivo

Objective:To study the effects of A549 cells proliferation with ADAM9 gene interference in vitro and in vivo.Methods:The expression of ADAM9 gene in A549 cells was detected by different methods.Proliferation and changes of A549 cells was observed using different concentrations of ADAM9 interference in vitro.Tumorigenicity was tested in nude mice after with different concentrations of ADAM9 gene interference A549.Results:ADAM9 major expression in A549 cells in the stroma and cytosol,less expression in cell membrane.Compared with control,A549 cell proliferative activity was significantly increased after human ADAM9 gene interference(P＜0.01).Tumorigenicity in nude mice was significantly promoted after interfering A549 by ADAM9 gene,compared with the control in vivo(P＜0.05).Conclusion:Both A549 cell proliferative activity in vitro and its tumorigenicity in vivo are promoted after ADAM9 gene interference.