人表皮生长因子受体2模拟表位的筛选

目的:从噬菌体12肽库中筛选出人表皮生长因子受体2（Her2）的抗原模拟表位。方法:以曲妥珠单抗为靶分子，在噬菌体12肽库中进行3轮淘选，以ELISA方法及竞争抑制实验鉴定阳性克隆，并对阳性克隆株进行测序。结果:经过3轮淘选，与曲妥珠单抗结合的噬菌体得到了有效富集，回收率从（2.00×10-8）%增加到（2.87×10-5）%，ELISA显示20个克隆中筛选获得了18个与曲妥珠单抗具有较高亲和性的阳性噬菌体，对阳性克隆测序获得两种氨基酸序列:HTSSLWHLFRST、VHWDFRQWWQPS。结论:噬菌体展示技术可成功筛选到表皮生长因子2模拟表位，为探索乳腺癌的防治研究创造了条件。

Screening and identification of the minotops of human epidermal growth factor receptor 2

Objective:To screen the minotops of Her2 from the phage display 12 peptide library.Methods:Used the Herceptin as the ligand to select the positive clones after 3 rounds of screening by the phage display 12 peptide library.The positive clones were identified by enzyme linked immunosorbent assay(ELISA) and competitive inhibition assay.The identified positive clones were analyzed by DNA sequencing.Results:The phages were effectively enriched after 3 rounds of planning with the improvement of the recovery rate was from(2.00×10-8)% to (2.87×10-5)%.In 20 individual phages,the 18 phages could combine the epidermal growth factor 2 monoclonal antibody.Two kinds of different sequences(HTSSLWHLFRST,VHWDFRQWWQPS) were obtained.Conclusion:The Her2 related antigen epitope is obtained by phage display technology.The result provides a new approach for breast cancer therapy and vaccine development.