汉防己甲素联合屈洛昔芬逆转K562/A02细胞耐药与诱导凋亡相关性的研究

本研究旨在探讨汉防己甲素(tetrandrine，Tet)联合屈洛昔芬(droloxifene，DRL)对耐药细胞系K562／A02的逆转作用与诱导凋亡有无相关性。采用Annexin V/PI法测定耐药调节剂汉防己甲素、屈洛昔芬单独或联合应用后细胞凋亡的情况。结果发现0.62ug/ml汉防己甲素或1/94ug/ml屈洛昔芬单独作用于K562细胞48小时后均可诱导一定比例的细胞凋亡，作用呈时间依赖性，两者联合应用作用增强。0.62ug/ml汉防己甲素或1.94ug/ml屈洛昔芬单独作用于K562／A02细胞均不能诱导细胞凋亡，两者联合应用72小时可诱导小部分细胞凋亡。结论：汉防己甲素，屈洛昔芬逆转耐药的机理与诱导K562／A02细胞凋亡无关。

Reversal Effect of Tetrandrine in Combination with Droloxifene on Cell Line K562/A02 and Its Correlation with Inducted Apoptosis

To study the correlation of the reversal effect of tetrandrine (Tet) in combination with droloxifene (DRL) on multidrug resistant cell line K562/A02 and induction apoptosis, the apoptosis of K562 cells and K562/A02 cells after the treatment of Tet and DRL alone or their combination was detected by flow cytometry (FCM). The results showed 0.62 microg/ml Tet and 1.94 microg/ml DRL alone could induce apoptosis of K562 cells in time-dependent manner after culture for 48 hours. Neither Tet at 0.62 microg/ml nor DRL at 1.94 microg/ml alone could induce apoptosis of K562/A02 cells, but in the combination of them two induced apoptosis of few K562/A02 cells in culture for 72 hours. In conclusion, the mechanism of Tet and DRL reversing multidrug resistance has no correlation with the apoptosis of K562/A02 cells.