急性脊髓损伤后线粒体形态结构与线粒体膜电位的变化

目的 探讨大鼠急性脊髓损伤后线粒体形态结构和线粒体膜电位的变化.方法 SD大鼠36只,随机分为对照组（假手术组）和脊髓损伤组（SCI组）,每组又分为处理后4h、8h、16h组（各6只）.SCI组采用Allen＆#39;s打击法,制作大鼠脊髓损伤模型,对照组只暴露脊髓,不打击.透射电镜观察线粒体形态结构的改变;在各时相提取伤段脊髓组织线粒体,JC-1法测定线粒体膜电位的变化.结果 SCI组脊髓组织伤后4h线粒体体积略增大,嵴排列稍紊乱;8h线粒体体积增大,嵴稍肿胀;16h线粒体结构不清,嵴断裂、排列紊乱,部分线粒体膜破裂,有空泡化.脊髓损伤后4h,线粒体膜电位开始明显下降（P＜0.01）,随着时间的延长,线粒体膜电位处于下降趋势.结论 大鼠急性脊髓损伤后线粒体的形态结构发生明显改变,伤段脊髓线粒体膜电位明显下降,出现功能障碍.

Changes of mitochondrial morphology and mitochondrial membrane potential following acute spinal cord injury

Objective To study the changes of mitochondrial morphology and mitochondrial membrane potential following acute spinal cord injury （SCI）. Methods Thirty-six SD rats were randomly divided into control group （sham operation group） and SCI group. Each group was divided into 4 h group, 8 h group and 16 h group after treatment （6 in each group）. The SCI group was established using Allen＇s method. Spinal cord of rats in control group was exposed. Changes of mitochondrial morphology were observed under TEM and mitochondrial membrane potential was measured using JC-1. Results The mitochondrial volume was larger with its crest deranged in SCI group 4 h after SCI. The mitochondrial volume was larger with its crest swollen in 8 h group. The mitochondrial structure was unclear with its crest broken and deranged, and some membrane ruptured and vacuole formed in 16 h group. The mitochondrial membrane potential was significantly lower in SCI group than in control group 4, 8 and 16 after SCI （P 〈 0.01）. Conclusion Significant change occurs in mitochondrial morphology and structure of rats with their mitochondrial membrane potential significantly decreases after SCI.